Art

J-GLOBAL ID：201402215566989172   Reference number：14A0437796

Construction of a pGreenBSK+plasmid vector with an inserted green fluorescence protein gene for use in experiments by students

学生実験のための緑色蛍光タンパク質遺伝子を組み込んだプラスミドベクターpGreen-BSK+の構築

Author (1)： KOBAYASHI YOKO (桐生大)
Material： 桐生大学紀要  (Bulletin of Kiryu University)
Issue： 24  Page： 103-110  Publication year： Dec. 20, 2013 
JST Material Number： L3093A  ISSN： 1342-4076  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： JAPANESE (JA)

Thesaurus term： vector(genetics) ,...
Semi thesaurus term： GFP gene ,...

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JST classification (1)： Gene manipulation  (EC02050B)

Reference (7)：

• 1 ) Shimomura, O., Johnson, F.H. et al: Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J. Cell Comp Physiol, 59: 223-239, 1962.
• 2 ) Chalfie, M: Green fluorescent protein. Photochem Photobiol, 62: 651-656, 1995.
• 3 ) Takada, T, Iida, K et al: Selective production of transgenic mice using green fluorescent protein as a marker. Nat Biotechnol, 15: 458-461, 1997.
• 4 ) Rizzuto, R., Brini, M. et al: Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells. Curr Biol, 5: 635-642, 1995.
• 5 ) Olson, K.R., McIntosh, J.R. et al: Analysis of MAP 4function in living cells using green fluorescent protein (GFP) chimeras. J. Cell Biol, 130: 639-650, 1995.

Terms in the title (5)： 学生実験 ,  緑色蛍光タンパク質 ,  遺伝子 ,  プラスミドベクター ,  構築