Art

J-GLOBAL ID：201402299083491920   Reference number：14A1262916

Effective Rho-associated protein kinase inhibitor treatment to dissociate human iPS cells for suspension culture to form embryoid body-like cell aggregates

胚様体様細胞集合体を形成させる懸濁培養に対するヒトiPS細胞を解離させるための効果的Rho関連蛋白質キナーゼ阻害剤処理

Author (5)： HORIGUCHI Ayumi ,  YAZAKI Koyuki ,  AOYAGI Mami ,  OHNUKI Yoshitsugu ,  KUROSAWA Hiroshi
Material： Journal of Bioscience and Bioengineering
Volume： 118  Issue： 5  Page： 588-592  Publication year： Nov. 2014 
JST Material Number： G0535B  ISSN： 1389-1723  CODEN： JBBIF6  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Thesaurus term： protein kinase ,...
Semi thesaurus term： circularity ,...

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JST classification (2)： Cell physiology in general  (EF02010S) ,  Development(=generation)and differentiation  (EJ16020W)

Substance index (1)： Y-27632

Reference (18)：

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• Kurosawa, H.: Methods for the formation of embryoid bodies: in vitro differentiation system of embryonic stem cells, J. Biosci. Bioeng., 103, 389-398 (2007).
• Ng, E. S., David, R. P., Azzola, L., Stanley, E. G., and Elefanty, A. G.: Forced aggregation of defined numbers of human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic differentiation, Blood, 106, 1601-1603 (2005).
• Koike, M., Sakaki, S., Amano, Y., and Kurosawa, H.: Characterization of embryoid bodies of mouse embryonic stem cells formed under various culture conditions and estimation of differentiation status of such bodies, J. Biosci. Bioeng., 104, 294-299 (2007).

Terms in the title (6)： 胚様体 ,  細胞集合体 ,  懸濁培養 ,  ヒトiPS細胞 ,  解離 ,  蛋白質キナーゼ阻害剤