Pat

J-GLOBAL ID：201403078823913685

効率的な人工多能性幹細胞の樹立方法

Inventor： 山中 伸弥 ,  高橋 和利 ,  沖田 圭介
Applicant, Patent owner： 国立大学法人京都大学
Agent (6)： 高島 一 ,  土井 京子 ,  鎌田 光宜 ,  田村 弥栄子 ,  山本 健二 ,  村田 美由紀
Gazette classification：特許公報
Application number (International application number)：2010506749
Patent number：5340265
Application date： Jun. 26, 2009
Claim (excerpt)：

【請求項1】体細胞の核初期化工程においてp53の機能を阻害することを含む、iPS細胞の樹立効率の改善方法であって、p53の機能の阻害が下記: (i) Pifithrin-αおよびその類縁体、Pifithrin-βおよびその類縁体、ならびにPifithrin-μ; (ii) p53のドミナントネガティブ変異体およびそれをコードする核酸; (iii) p53に対するsiRNA、shRNAおよびそれらをコードするDNA; (iv) MDM2およびそれをコードする核酸;ならびに (v) p21に対するsiRNA、shRNA、アンチセンス核酸およびリボザイム からなる群から選択される1またはそれ以上の物質により行われる、方法。

IPC (3)：

C12N 5/10 ( 200 6.01) ,  C12N 15/09 ( 200 6.01) ,  C12N 15/113 ( 201 0.01)

FI (3)：

C12N 5/00 102 ,  C12N 15/00 ZNA A ,  C12N 15/00 G

Article cited by the Patent：

Cited by applicant (12)

• The R246S hot-spot p53 mutant exerts dominant-negative effects in embryonic stem cells in vitro and
• Knockdown of p53 by RNAi in ES cells facilitates RA-induced differentiation into muscle cells.
• Generation of cloned mice by direct nuclear transfer from natural killer T cells.
• Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal
• Two supporting factors greatly improve the efficiency of human iPSC generation.
• Human telomerase catalytic subunit (hTERT) suppresses p53-mediated anti-apoptotic response via induc
• From fibroblasts to iPS cells: induced pluripotency by defined factors.
• A p53-mediated DNA damage response limits reprogramming to ensure iPS cell genomic integrity.
• Linking the p53 tumour suppressor pathway to somatic cell reprogramming.
• Suppression of induced pluripotent stem cell generation by the p53-p21 pathway.
• Overexpression of BMI1 confers clonal cells resistance to apoptosis and contributes to adverse progn
• Opposing effects of Ras on p53: transcriptional activation of mdm2 and induction of p19ARF.

Cited by examiner (12)

• The R246S hot-spot p53 mutant exerts dominant-negative effects in embryonic stem cells in vitro and
• Knockdown of p53 by RNAi in ES cells facilitates RA-induced differentiation into muscle cells.
• Generation of cloned mice by direct nuclear transfer from natural killer T cells.
• Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal
• Two supporting factors greatly improve the efficiency of human iPSC generation.
• Human telomerase catalytic subunit (hTERT) suppresses p53-mediated anti-apoptotic response via induc
• From fibroblasts to iPS cells: induced pluripotency by defined factors.
• A p53-mediated DNA damage response limits reprogramming to ensure iPS cell genomic integrity.
• Linking the p53 tumour suppressor pathway to somatic cell reprogramming.
• Suppression of induced pluripotent stem cell generation by the p53-p21 pathway.
• Overexpression of BMI1 confers clonal cells resistance to apoptosis and contributes to adverse progn
• Opposing effects of Ras on p53: transcriptional activation of mdm2 and induction of p19ARF.