Art

J-GLOBAL ID：201502207355632265   Reference number：15A0875760

Dual labeling with 5-bromo-2’-deoxyuridine and 5-ethynyl-2’-deoxyuridine for estimation of cell migration rate in the small intestinal epithelium

小腸上皮における細胞遊走速度推定のための5-ブロモ-2′-デオキシウリジンと5-エチニル-2′-デオキシウリジンによる二重標識

Author (5)： ASANO Mami (Graduate School of Life Sci., Hokkaido Univ., Sapporo, 060-8589, JPN) ,  YAMAMOTO Tatsuro ,  TSURUTA Takeshi ,  NISHIMURA Naomichi ,  SONOYAMA Kei
Material： Development, Growth & Differentiation  (Dev Growth Differ)
Volume： 57  Issue： 1  Page： 68-73  Publication year： Jan. 2015 
JST Material Number： W0832A  ISSN： 0012-1592  Document type： Article
Article type： 原著論文  Country of issue： United States (USA)  Language： ENGLISH (EN)

Thesaurus term： rat ,...
Semi thesaurus term： click reaction ,...

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JST classification (3)： Intestines  (EJ06040N) ,  Cell physiology in general  (EF02010S) ,  Microscopic methods of living organisms  (EA03050C)

Substance index (2)： 5-Ethynyl-2'-deoxyuridine ,  Bromodeoxyuridine

Reference (14)：

• Buck, S. B., Bradford, J., Gee, K. R., Agnew, B. J., Clarke, S. T. & Salic, A. 2008. Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2'-deoxyuridine antibodies. Biotechniques 44, 927-929.
• Cappella, P., Gasparri, F., Pulici, M. & Moll, J. 2008. A novel method based on click chemistry, which overcomes limitations of cell cycle analysis by classical determination of BrdU incorporation, allowing multiplex antibody staining. Cytometry A. 73, 626-636.
• van der Flier, L. G. & Clevers, H. 2009. Stem cells, self-renewal, and differentiation in the intestinal epithelium. Annu. Rev. Physiol. 71, 241-260.
• Hsu, T. L., Hanson, S. R., Kishikawa, K., Wang, S. K., Sawa, M. & Wong, C. H. 2007. Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells. Proc. Natl Acad. Sci. USA 104, 2614-2619.
• Ito, H., Satsukawa, M., Arai, E., Sugiyama, K., Sonoyama, K., Kiriyama, S. & Morita, T. 2009. Soluble fiber viscosity affects both goblet cell number and small intestine mucin secretion in rats. J. Nutr. 139, 1640-1647.

Terms in the title (5)： 腸上皮 ,  細胞遊走 ,  速度推定 ,  5-ブロモ-2′-デオキシウリジン ,  5-エチニル-2′-デオキシウリジン