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J-GLOBAL ID：201502221285020480   Reference number：15A0791167

A hyperactive piggyBac transposon system is an easy-to-implement method for introducing foreign genes into mouse preimplantation embryos

高活性piggyBacトランスポゾンシステムは,外来遺伝子をマウス着床前胚に導入するための簡単に実施できる方法である

Author (5)： SUZUKI Shinnosuke (Lab. of Reproductive Biology, Graduate School of Agriculture, Kyoto Univ., Kyoto 606-8502, JPN) ,  TSUKIYAMA Tomoyuki (Res. Center for Animal Life Sci., Shiga Univ. of Medical Sci., Shiga 520-2192, JPN) ,  KANEKO Takehito (Inst. of Lab. Animals, Graduate School of Medicine, Kyoto Univ., Kyoto 606-8501, JPN) ,  IMAI Hiroshi (Lab. of Reproductive Biology, Graduate School of Agriculture, Kyoto Univ., Kyoto 606-8502, JPN) ,  MINAMI Naojiro (Lab. of Reproductive Biology, Graduate School of Agriculture, Kyoto Univ., Kyoto 606-8502, JPN)
Material： Journal of Reproduction and Development  (J Reprod Dev)
Volume： 61  Issue： 3  Page： 241-244 (J-STAGE)  Publication year： 2015 
JST Material Number： F0766A  ISSN： 0916-8818  Document type： Article
Article type： 解説  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *transposon ,...
Semi thesaurus term： recombination ,...

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JST classification (3)： Laboratry organisms  (EA03070Y) ,  Gene manipulation  (EC02050B) ,  Development(=generation)and differentiation  (EJ16020W)

Reference (15)：

• 1. Nakanishi T, Kuroiwa A, Yamada S, Isotani A, Yamashita A, Tairaka A, Hayashi T, Takagi T, Ikawa M, Matsuda Y, Okabe M. FISH analysis of 142 EGFP transgene integration sites into the mouse genome. Genomics 2002; 80: 564-574.
• 2. Ittner LM, Götz J. Pronuclear injection for the production of transgenic mice. Nat Protoc 2007; 2: 1206-1215.
• 3. Sumiyama K, Kawakami K, Yagita K. A simple and highly efficient transgenesis method in mice with the Tol2 transposon system and cytoplasmic microinjection. Genomics 2010; 95: 306-311.
• 4. Takeda J, Keng VW, Horie K. Germline mutagenesis mediated by Sleeping Beauty transposon system in mice. Genome Biol 2007; 8(Suppl 1): S14.
• 5. Marh J, Stoytcheva Z, Urschitz J, Sugawara A, Yamashiro H, Owens JB, Stoytchev I, Pelczar P, Yanagimachi R, Moisyadi S. Hyperactive self-inactivating piggyBac for transposase-enhanced pronuclear microinjection transgenesis. Proc Natl Acad Sci USA 2012; 109: 19184-19189.

Terms in the title (6)： 活性 ,  piggyBac ,  トランスポゾン ,  外来遺伝子 ,  マウス ,  着床前胚