Art
J-GLOBAL ID：201502236259457460   Reference number：15A0227119

Expression of bioactive soluble human stem cell factor (SCF) from recombinant Escherichia coli by coproduction of thioredoxin and efficient purification using arginine in affinity chromatography

組換大腸菌のチオレドキシン同時生成による生物活性可溶性ヒト幹細胞因子(SCF)の発現とアルギニンを用いたアフィニティークロマトグラフィーによる効率的精製
Author (12)：
AKUTA Teruo

About AKUTA Teruo

(Teikyo Univ. School of Medicine, Tokyo, JPN)

About Teikyo Univ. School of Medicine, Tokyo, JPN

, 
AKUTA Teruo

About AKUTA Teruo

(Kyokuto Pharmaceutical Industrial Co. Ltd., Tokyo, JPN)

About Kyokuto Pharmaceutical Industrial Co. Ltd., Tokyo, JPN

, 
KIKUCHI-UEDA Takane

About KIKUCHI-UEDA Takane

(Teikyo Univ. School of Medicine, Tokyo, JPN)

About Teikyo Univ. School of Medicine, Tokyo, JPN

, 
IMAIZUMI Keitaro

About IMAIZUMI Keitaro

(Teikyo Univ. School of Medicine, Tokyo, JPN)

About Teikyo Univ. School of Medicine, Tokyo, JPN

, 
IMAIZUMI Keitaro

About IMAIZUMI Keitaro

(Kyokuto Pharmaceutical Industrial Co. Ltd., Tokyo, JPN)

About Kyokuto Pharmaceutical Industrial Co. Ltd., Tokyo, JPN

, 
OSHIKANE Hiroyuki

About OSHIKANE Hiroyuki

(Teikyo Univ. School of Medicine, Tokyo, JPN)

About Teikyo Univ. School of Medicine, Tokyo, JPN

, 
NAKAKI Toshio

About NAKAKI Toshio

(Teikyo Univ. School of Medicine, Tokyo, JPN)

About Teikyo Univ. School of Medicine, Tokyo, JPN

, 
OKADA Yoko

About OKADA Yoko

(National Res. Inst. for Child Health and Dev., Tokyo, JPN)

About National Res. Inst. for Child Health and Dev., Tokyo, JPN

, 
SULTANA Sara

About SULTANA Sara

(National Res. Inst. for Child Health and Dev., Tokyo, JPN)

About National Res. Inst. for Child Health and Dev., Tokyo, JPN

, 
KOBAYASHI Kenichiro

About KOBAYASHI Kenichiro

(National Res. Inst. for Child Health and Dev., Tokyo, JPN)

About National Res. Inst. for Child Health and Dev., Tokyo, JPN

, 
KIYOKAWA Nobutaka

About KIYOKAWA Nobutaka

(National Res. Inst. for Child Health and Dev., Tokyo, JPN)

About National Res. Inst. for Child Health and Dev., Tokyo, JPN

, 
ONO Yasuo

About ONO Yasuo

(Teikyo Univ. School of Medicine, Tokyo, JPN)

About Teikyo Univ. School of Medicine, Tokyo, JPN


Material：
Protein Expression and Purification  (Protein Expression & Purification)

About Protein Expression and Purification


Volume： 105  Page： 1-7  Publication year： Jan. 2015 
JST Material Number： W0282A  ISSN： 1046-5928  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)
Thesaurus term：
Thesaurus term/Semi thesaurus term
Keywords indexed to the article.
All keywords is available on JDreamIII(charged).
On J-GLOBAL, this item will be available after more than half a year after the record posted. In addtion, medical articles require to login to MyJ-GLOBAL.
*SCF

About SCF

, 
recombinant protein

About recombinant protein

, 
*protein synthesis

About protein synthesis

, 
Escherichia coli

About Escherichia coli

, 
thioredoxin

About thioredoxin

, 
*affinity chromatography

About affinity chromatography

, 
protein

About protein

, 
human(primates)

About human(primates)

, 
fusion protein

About fusion protein

, 
biological activity

About biological activity

, 
amino acid

About amino acid

, 
aliphatic amine

About aliphatic amine

, 
aliphatic carboxylic acid

About aliphatic carboxylic acid

, 
primary amine

About primary amine


Semi thesaurus term：
Thesaurus term/Semi thesaurus term
Keywords indexed to the article.
All keywords is available on JDreamIII(charged).
On J-GLOBAL, this item will be available after more than half a year after the record posted. In addtion, medical articles require to login to MyJ-GLOBAL.
*Recombinant protein expression

About Recombinant protein expression

, 
soluble protein

About soluble protein

, 
coexpression

About coexpression


JST classification (2)：
JST classification
Category name(code) classified by JST.
Cytokines 
(EB08090G)

About Cytokines

,  Microbial metabolite production 
(FK03020A)

About Microbial metabolite production

Substance index (1)：
Substance index
Chemical Substance indexed to the Article.
Arginine

About Arginine

Terms in the title (11)：
Terms in the title
Keywords automatically extracted from the title.
組換大腸菌

About 組換大腸菌

, 
チオレドキシン

About チオレドキシン

, 
生物活性

About 生物活性

, 
可溶性

About 可溶性

, 
ヒト

About ヒト

, 
幹細胞因子

About 幹細胞因子

, 
SCF

About SCF

, 
発現

About 発現

, 
アルギニン

About アルギニン

, 
アフィニティークロマトグラフィー

About アフィニティークロマトグラフィー

, 
精製

About 精製


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