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J-GLOBAL ID：201602208755360802   Reference number：16A0106150

Direct coupling of immobilized metal ion affinity chromatography and capillary isoelectric focusing in a single capillary

単一キャピラリーでの固定化金属イオンアフィニティークロマトグラフィーとキャピラリー等電点電気泳動の直接カップリング
Author (2)：
SHIMURA Kiyohito

About SHIMURA Kiyohito

(Div. of Advanced Chemistry, School of Medicine, Fukushima Medical Univ.)

About Div. of Advanced Chemistry, School of Medicine, Fukushima Medical Univ.

, 
NAGAI Toshihiko

About NAGAI Toshihiko

(Div. of Advanced Chemistry, School of Medicine, Fukushima Medical Univ.)

About Div. of Advanced Chemistry, School of Medicine, Fukushima Medical Univ.


Material：
Journal of Electrophoresis (Web)  (J Electrophor (Web))

About Journal of Electrophoresis (Web)


Volume： 59  Issue： 1  Page： 7-17 (J-STAGE)  Publication year： 2015 
JST Material Number： U0263A  ISSN： 1349-9408  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)
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*affinity chromatography

About affinity chromatography

,...
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IMAC(chromato)

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JST classification (2)：
JST classification
Category name(code) classified by JST.
Biophysical research methods 
(EA03020V)

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,  Proteins and peptides in general 
(EB03010N)

About Proteins and peptides in general

Reference (26)：
  • 1) Hjerten S, Zhu MD. Adaptation of the Equipment for High-Performance Electrophoresis to Isoelectric Focusing. J Chromatogr. 1985;346:265-270.
  • 2) Silvertand LH, Torano JS, van Bennekom WP, et al. Recent developments in capillary isoelectric focusing. J Chromatogr A. 2008;1204:157-170.
  • 3) Shimura K. Recent advances in IEF in capillary tubes and microchips. Electrophoresis. 2009;30:11-28.
  • 4) Chen JQ, Lee JH, Herrmann MA, et al. Capillary isoelectric-focusing immunoassays to study dynamic oncoprotein phosphorylation and drug response to targeted therapies in non-small cell lung cancer. Mol Cancer Ther. 2013;12:2601-2613.
  • 5) Rustandi RR, Peklansky B, Anderson CL. Use of imaged capillary isoelectric focusing technique in development of diphtheria toxin mutant CRM197. Electrophoresis. 2014;35:1065-1071.
ｍore...
Terms in the title (5)：
Terms in the title
Keywords automatically extracted from the title.
キャピラリー

About キャピラリー

, 
固定化

About 固定化

, 
金属イオンアフィニティークロマトグラフィー

About 金属イオンアフィニティークロマトグラフィー

, 
キャピラリー等電点電気泳動

About キャピラリー等電点電気泳動

, 
カップリング

About カップリング


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