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J-GLOBAL ID：201602278757670657   Reference number：16A1051675

A simple agroinfiltration method for transient gene expression in plant leaf discs

植物葉のディスクにおける一過性遺伝子発現のための簡易なアグロ溶浸法

Author (3)： Matsuo Kouki (Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan) ,  Fukuzawa Noriho (Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan) ,  Matsumura Takeshi (Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan)
Material： Journal of Bioscience and Bioengineering
Volume： 122  Issue： 3  Page： 351-356  Publication year： Sep. 2016 
JST Material Number： G0535B  ISSN： 1389-1723  CODEN： JBBIF6  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Thesaurus term： *gene expression ,...
Semi thesaurus term： *Agrobacterium ,...

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Author keywords (5)： Key words Transient gene expression ,  Vacuum infiltration ,  Leaf disc ,  Agroinfiltration ,  Nicotiana benthamiana

JST classification (4)： Crop breeding  (FC02030G) ,  Microorganism physiology in general  (EG03051L) ,  Gene expression  (EC02040Q) ,  Research methods in biological sciences in general  (EA03010K)

Reference (21)：

• Kapila, J., DeRycke, R., VanMontagu, M., and Angenon, G.: An Agrobacterium-mediated transient gene expression system for intact leaves, Plant Sci., 122, 101-108 (1997).
• Gleba, Y., Klimyuk, V., and Marillonnet, S.: Viral vectors for the expression of proteins in plants, Curr. Opin. Biotechnol., 18, 134-141 (2007).
• Hefferon, K. L.: Plant virus expression vectors set the stage as production platforms for biopharmaceutical proteins, Virology, 433, 1-6 (2012).
• Matsuo, K., Hong, J. S., Tabayashi, N., Ito, A., Masuta, C., and Matsumura, T.: Development of Cucumber mosaic virus as a vector modifiable for different host species to produce therapeutic proteins, Planta, 225, 277-286 (2007).
• Wang, Y. C., Klein, T. M., Fromm, M., Cao, J., Sanford, J. C., and Wu, R.: Transient expression of foreign genes in rice, wheat and soy-bean cells following particle bombardment, Plant Mol. Biol., 11, 433-439 (1988).

Terms in the title (4)： 植物葉 ,  ディスク ,  遺伝子発現 ,  溶浸法