Nakade Shota

Affiliation and department：

Research field (1)： Systems genomics

Research keywords (5)： Cultured cell , TALEN , CRISPR-Cas9 , knock-in , genome editing

Research theme for competitive and other funds (6)：

2021 - 2022 Gene therapy by precise single nucleotide modification using genome editing tool
2019 - 2021 Development of intercellular device to record the extracellular stimuli with self-targeting CRISPR-Cas9
2016 - 2019 CRISPRスクリーニングによるがん微小環境下での浸潤関連遺伝子の網羅的解析
2016 - 2017 ゲノム編集技術を用いた簡便・正確・高効率なSNP改変法の開発
2015 - 2016 ゲノム編集法によるMMEJを用いた汎用的かつ高効率な遺伝子ノックイン法の確立

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Papers (10)：

Nakade, Shota, Mochida, Keiji, Kunii, Atsushi, Nakamae, Kazuki, Aida, Tomomi, Tanaka, Kohichi, Sakamoto, Naoaki, Sakuma, Tetsushi, Yamamoto, Takashi. Biased genome editing using the local accumulation of DSB repair molecules system. NATURE COMMUNICATIONS. 2018. 9. 1. 3270-3270
Sakuma, Tetsushi, Mochida, Keiji, Nakade, Shota, Ezure, Toru, Minagawa, Sachi, Yamamoto, Takashi. Unexpected heterogeneity derived from Cas9 ribonucleoprotein-introduced clonal cells at the HPRT1 locus. GENES TO CELLS. 2018. 23. 4. 255-263
Nakade, Shota, Yamamoto, Takashi, Sakuma, Tetsushi. Cancer induction and suppression with transcriptional control and epigenome editing technologies. JOURNAL OF HUMAN GENETICS. 2018. 63. 2. 187-194
Shota Nakade, Takashi Yamamoto, Tetsushi Sakuma. Cas9, Cpf1 and C2c1/2/3-What's next?. BIOENGINEERED. 2017. 8. 3. 265-273
Kazuki Nakamae, Yuki Nishimura, Mitsumasa Takenaga, Shota Nakade, Naoaki Sakamoto, Hiroshi Ide, Tetsushi Sakuma, Takashi Yamamoto. Establishment of expanded and streamlined pipeline of PITCh knock-in - a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO. BIOENGINEERED. 2017. 8. 3. 302-308

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MISC (13)：

佐久間哲史, 中出翔太. ゲノム編集ツールの開発動向と関連技術ゲノム・エピゲノム編集ツール 1.新規ゲノム編集ツールの開発動向. 実験医学. 2016. 34. 20. 3270-3275
佐久間哲史, 中出翔太, 山本卓. ゲノム編集ツールの開発動向と関連技術最新CRISPR関連アプリケーション 8.さまぎまな遺伝子ノックインシステム. 実験医学. 2016. 34. 20. 3321-3327
Tomomi Aida, Tetsushi Sakuma, Shota Nakade, Takashi Yamamoto, Kohichi Tanaka. Gene cassette knock-in in mice with cloning-free CRISPR/Cas system. TRANSGENIC RESEARCH. 2016. 25. 2. 215-216
中出翔太, 佐久間哲史, 持田圭次, 大石鮎, 山本卓. MMEJ修復経路の活性化によるヒト培養細胞での遺伝子ノックインの効率化. 日本分子生物学会年会プログラム・要旨集(Web). 2016. 39th. ROMBUNNO.1P-0806 (WEB ONLY)
佐久間哲史, 中出翔太, 坂根祐人, 鈴木賢一, 山本卓. ゲノム編集で細胞・生物をカスタマイズする改良された標的染色体への精密な統合(PITCh)法微小相同性を介した末端結合(MMEJ)を介した遺伝子ノックインに関する利便性、効率、および応用可能性の改善(Improved PITCh systems: enhancing convenience, efficiency, and applicability of MMEJ-mediated gene knock-in). 日本生化学会大会・日本分子生物学会年会合同大会講演要旨集. 2015. 88回・38回. [3S14-4]

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Books (3)：

Lectures and oral presentations (1)：

Education (3)：

2014 - 2016 Hiroshima University Graduate School of Science Mathematical and Life Science
2012 - 2014 Hiroshima University Graduate School of Science Biological Science
2008 - 2012 Hiroshima University School of Science Biological Science

Work history (2)：

2019/04 - 現在 Massachusetts Institute of Technology Synthetic biology center
2016/04 - 2019/03 Hiroshima university Graduate School of Science Mathematical and Life Science

Awards (2)：

Association Membership(s) (2)：

THE MOLECULAR BIOLOGY SOCIETY OF JAPAN , the japanese society of genome editing

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