Rchr
J-GLOBAL ID：201701017533525149
Update date: Mar. 26, 2024
Shintomi Keishi
シントミ ケイシ | Shintomi Keishi
Affiliation and department：
Job title：
Senior Research Scientist (tenured)
Research field (3)：
Functional biochemistry
, Molecular biology
, Cell biology
Research keywords (10)：
nucleosome
, topoisomerase
, condensin
, fertilization
, Xenopus egg extract
, reconstitution
, chromatin
, Xenopus laevis
, cell cycle
, chromosome
Research theme for competitive and other funds (9)：
- 2022 - 2025 Roles for nucleosomes and DNA topology in mitotic chromosome assembly
- 2019 - 2024 Reconstitution of totipotent nuclei in a test tube
- 2018 - 2021 In-vitro reconstitution of mitotic chromosomes
- 2016 - 2018 Roles for histones in assembly of mitotic chromosomes
- 2014 - 2017 Reconstitution of mitotic chromosomes with purified factors
- 2014 - 2016 Roles for nucleosomes in assembly of mitotic chromosomes
- 2012 - 2014 Reconstitution of mitotic chromatids with a minimum set of purified proteins
- 2010 - 2012 Molecular dissection of sister chromatid cohesion
- 2009 - Biochemical dissection of sister chromatid cohesion
Show all
Papers (20)：
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Makoto M. Yoshida, Kazuhisa Kinoshita, Keishi Shintomi, Yuuki Aizawa, Tatsuya Hirano. Regulation of condensin II by self-suppression and release mechanisms. Molecular Biology of the Cell. 2023
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Keishi Shintomi, Yuki Masahara-Negishi, Masami Shima, Shoji Tane, Tatsuya Hirano. Recombinant cyclin B-Cdk1-Suc1 capable of multi-site mitotic phosphorylationin vitro. PLOS ONE. 2023. 19. 3. e0299003
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Mingxuan Sun, Hossein Amiri, Alexander B Tong, Keishi Shintomi, Tatsuya Hirano, Carlos Bustamante, Rebecca Heald. Monitoring the compaction of single DNA molecules in Xenopus egg extract in real time. Proceedings of the National Academy of Sciences of the United States of America. 2023. 120. 12. e2221309120
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Yuko Fukuda, Keishi Shintomi, Kosuke Yamaguchi, Yasuhiro Fujiwara, Yuki Okada. Solubilization of Mouse Sperm Chromatin for Sequencing Analyses Using a Chaperon Protein. Methods in molecular biology (Clifton, N.J.). 2023. 2577. 161-173
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Shoji Tane, Keishi Shintomi, Kazuhisa Kinoshita, Yuko Tsubota, Makoto M Yoshida, Tomoko Nishiyama, Tatsuya Hirano. Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit. eLife. 2022. 11
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MISC (6)：
Lectures and oral presentations (20)：
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Reconstitution of mitotic chromosomes for studying their physicochemical properties
(2022)
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Biochemical dissection of nuclear reprogramming on somatic cell nuclear transfer
(The International Symposium "Totipotency and Germ Cell Development" 2022)
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Encapsulation of Xenopus egg extract into giant liposomes by phase-transfer of inverted emulsions
(2022)
-
カエル卵を使って見えてきた染色体の構築メカニズム
(第93回日本動物学会大会 サテライトシンポジウム 2022)
-
Making a chromosome in a test tube
(RIKEN BDR seminar (RIKEN Yokohama institute) 2022)
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Education (2)：
- 2000 - 2005 Tokyo Institute of Technology Graduate School of Bioscience
- 1996 - 2000 Tokyo Institute of Technology Faculty of Bioscience
Professional career (1)：
- Ph.D. (Tokyo Institute of Technology)
Work history (5)：
- 2018/04 - 現在 RIKEN Senior Research Scientist (tenured)
- 2011/04 - 2018/03 RIKEN Research Scientist (tenured)
- 2010/04 - 2011/03 RIKEN Postdoctoral Fellow
- 2007/04 - 2010/03 RIKEN Special Postdoctoral Fellow (fellowship awarded)
- 2005/10 - 2007/03 Cold Spring Harbor Laboratory Postdoctoral fellow
Association Membership(s) (3)：
JAPAN SOCIETY FOR CELL BIOLOGY
, THE MOLECULAR BIOLOGY SOCIETY OF JAPAN
, 日本生化学会
