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J-GLOBAL ID：201702220151137028   Reference number：17A0566016

Optimization of Albumin Secretion and Metabolic Activity of Cytochrome P450 1A1 of Human Hepatoblastoma HepG2 Cells in Multicellular Spheroids by Controlling Spheroid Size

多細胞スフェロイドにおけるヒト肝芽腫HepG2細胞のアルブミン分泌とシトクロムP450 1A1の代謝活性のスフェロイドサイズの制御による最適化

Author (17)： Nishikawa Tomoko (Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University) ,  Tanaka Yutaro (Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University) ,  Nishikawa Makiya (Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University) ,  Nishikawa Makiya (Institute for Innovative NanoBio Drug Discovery and Development Graduate School of Pharmaceutical Sciences, Kyoto University) ,  Ogino Yuka (Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University) ,  Kusamori Kosuke (Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University) ,  Mizuno Narumi (Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University) ,  Mizukami Yuya (Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University) ,  Shimizu Kazunori (Institute for Innovative NanoBio Drug Discovery and Development Graduate School of Pharmaceutical Sciences, Kyoto University) ,  Shimizu Kazunori (Department of Biotechnology, Graduate School of Engineering, Nagoya University) ,  Shimizu Kazunori (Department of Mechanical Engineering, Ritsumeikan University) ,  Konishi Satoshi (Institute for Innovative NanoBio Drug Discovery and Development Graduate School of Pharmaceutical Sciences, Kyoto University) ,  Konishi Satoshi (Department of Mechanical Engineering, Ritsumeikan University) ,  Konishi Satoshi (Ritsumeikan-Global Innovation Research Organization, Ritsumeikan University) ,  Takahashi Yuki (Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University) ,  Takakura Yoshinobu (Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University) ,  Takakura Yoshinobu (Institute for Innovative NanoBio Drug Discovery and Development Graduate School of Pharmaceutical Sciences, Kyoto University)
Material： Biological and Pharmaceutical Bulletin  (Biological & Pharmaceutical Bulletin)
Volume： 40  Issue： 3  Page： 334-338(J-STAGE)  Publication year： 2017 
JST Material Number： S0989A  ISSN： 0918-6158  CODEN： BPBLEO  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： cultured cell ,...
Semi thesaurus term： cell physiology ,...

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Author keywords (10)： albumin ,  CYP1A1 ,  human hepatoblastoma HepG2 cell ,  size control ,  spheroid ,  albumin ,  CYP1A1 ,  human hepatoblastoma HepG2 cell ,  size control ,  spheroid

JST classification (1)： Cell and tissue culture methods  (EA03040R)

Reference (40)：

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• 3) Torisawa YS, Takagi A, Nashimoto Y, Yasukawa T, Shiku H, Matsue T. A multicellular spheroid array to realize spheroid formation, culture, and viability assay on a chip. Biomaterials, 28, 559-566 (2007).
• 4) Vinci M, Gowan S, Boxall F, Patterson L, Zimmermann M, Court W, Lomas C, Mendiola M, Hardisson D, Eccles SA. Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation. BMC Biol., 10, 29 (2012).
• 5) Godugu C, Patel AR, Desai U, Andey T, Sams A, Singh M. AlgiMatrixTM based 3D cell culture system as an in-vitro tumor model for anticancer studies. PLoS ONE, 8, e53708 (2013).

Terms in the title (11)： 多細胞スフェロイド ,  ヒト ,  肝芽腫 ,  HepG2細胞 ,  アルブミン ,  分泌 ,  シトクロムP450 ,  代謝活性 ,  スフェロイド ,  サイズ ,  最適化