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J-GLOBAL ID：201702243673028787   Reference number：17A0962094

Glass Bead-based Genetic Transformation:An Efficient Method for Transformation of Thraustochytrid Microorganisms

ガラスビーズを基にした遺伝的形質転換:thraustochytrid微生物の形質転換のための効率的な一方法

Author (6)： Adachi Takumi (Laboratory of Molecular Environmental Microbiology, Graduate School of Agriculture, Hokkaido University) ,  Adachi Takumi (Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)) ,  Sahara Takehiko (Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)) ,  Okuyama Hidetoshi (Laboratory of Environmental Molecular Biology, Section of Environmental Biology, Faculty of Environmental Earth Science, Hokkaido University) ,  Morita Naoki (Laboratory of Molecular Environmental Microbiology, Graduate School of Agriculture, Hokkaido University) ,  Morita Naoki (Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST))
Material： Journal of Oleo Science  (J Oleo Sci)
Volume： 66  Issue： 7  Page： 791-795(J-STAGE)  Publication year： 2017 
JST Material Number： G0238A  ISSN： 1345-8957  CODEN： JOSOAP  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： glass bead ,...
Semi thesaurus term： Aurantiochytrium limacinum ,...

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Author keywords (4)： glass bead treatment ,  electroporation ,  genetic transformation ,  thraustochytrid

JST classification (2)： Gene manipulation  (EC02050B) ,  Microbiology(other than viruses)in general  (EG03010C)

Substance index (1)： Docosahexaenoic acid

Reference (12)：

• 1) Lewis, T.E.; Nichols, P.D.; McMeekin, T.A. The biotechnologyical potential of thraustochytrids. Mar. Biotechnol. 1, 580-587 (1999).
• 2) Kaya, K.; Nakazawa, A.; Matsuura, H.; Honda, D.; Inouye, I.; Watanabe, M. Thraustochytrid Aurantiochytrium sp. 18W-13a accumulates high amounts of squalene. Biosci. Biotechnol. Biochem. 75, 2246-2248 (2011).
• 3) Aki, T.; Hachida, K.; Yoshinaga, M.; Katai, Y.; Yamasaki, T.; Kawamoto, S.; Kakizono, T.; Maoka, T.; Shigeta, S.; Suzuki, O.; Ono, K. Thraustochytrid as a potential source of caroteneoids. J. Am. Oil Chem. Soc. 80, 789-794 (2003).
• 4) Sakaguchi, K.; Matsuda, T.; Kobayashi, T.; Ohara, J.; Hamaguchi, R.; Abe, E.; Nagano, N.; Hayashi, M.; Ueda, M.; Honda, D.; Okita, Y.; Taoka, Y.; Sugimoto, S.; Okino, N.; Ito, M. Versatile transformation system that is applicable to both multiple transgene expression and gene targeting for thraustochytrids. Appl. Environ. Microbiol. 78, 3193-3202 (2012).
• 5) Cheng, R.; Ma, R.; Li, K.; Rong, H.; Lin, X.; Wang, Z.; Yang, S.; Ma, Y. Agrobacterium tumefaciens mediated transformation of marine microalgae Schizochytrium. Microbiol. Res. 167, 179-186 (2012).

Terms in the title (6)： ガラス ,  ビーズ ,  遺伝的形質転換 ,  微生物 ,  形質転換 ,  法