Art

J-GLOBAL ID：201702254686771054   Reference number：17A0283983

A Culture Method of Rat Hepatocytes Using the Three-dimensional Cell Culture Scaffold “Cellbed” and Its Application for Liver Toxicity Testing

三次元細胞培養足場”Cellbed”を用いたラット肝細胞の培養手法と肝臓毒性試験へのその応用

Author (6)： IWASA Takuya (Japan Vilene Co., Ltd., Ibaraki, JPN) ,  WATANABE Rie (Japan Vilene Co., Ltd., Ibaraki, JPN) ,  SASAKI Kouhei (Japan Vilene Co., Ltd., Ibaraki, JPN) ,  MATSUSHITA Taku (Sojo Univ., Kumamoto, JPN) ,  YAMAGUCHI Akihiro (Cosmo Bio Co., Ltd., Sapporo, JPN) ,  KAWABE Masaaki (Japan Vilene Co., Ltd., Ibaraki, JPN)
Material： Alternatives to Animal Testing and Experimentation  (AATEX)
Volume： 21  Issue： 2  Page： 52-62  Publication year： Dec. 31, 2016 
JST Material Number： L5507A  ISSN： 1344-0411  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： rat ,...
Semi thesaurus term： Hepatic metabolism ,...

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JST classification (1)： Cell and tissue culture methods  (EA03040R)

Reference (15)：

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• Ben-Ze'ev A., Robinson S.G., Bucher L.R.N., and Farmer R.S. (1988) Cell-cell and cell-matrix interactions differentially regulate the expression of hepatic and cytoskeletal genes in primary cultures of rat hepatocytes, Proc. Nati. Acad. Sci. U.S.A., 85, 2161-2165.
• Bissell D.M., Arenson M.D., Maher J.J., and Roll F. J. (1987) Support of Cultured Hepatocytes by a Laminin-rich gel. Evidence for a Functionally Significant Subendothelial Matrix in Normal Rat Liver, J. Clin. Invest., 79, 801-812.
• Caron M.J. (1990) Induction of Albumin Gene Transcription in Hepatocytes by Extracellular Matrix Proteins, Mol. Cell. Biol., 10(3), 1239-1243.
• Dipersio C.M., Jackson A.D., and Zarat S.K. (1991) The Extracellular Matrix Coordinately Modulates Liver Transcription Factors and Hepatocyte Morphology, Mol. Cell Biol., 11(9), 4405-4414.

Terms in the title (9)： 三次元細胞培養 ,  足場 ,  ラット ,  肝細胞 ,  培養 ,  手法 ,  肝臓毒性 ,  試験 ,  応用