Art
J-GLOBAL ID：201702255392687031   Reference number：17A0470644

One-step combined focused epPCR and saturation mutagenesis for thermostability evolution of a new cold-active xylanase

新しい低温活性キシラナーゼの熱安定性進化のための一段階複合集束epPCRと飽和変異誘発【Powered by NICT】
Author (6)：
Acevedo Juan Pablo

About Acevedo Juan Pablo

(Facultad de Medicina y Facultad de Ingenieria y Ciencias Aplicadas, Universidad de los Andes, San Carlos de Apoquindo, 2200 Santiago, Chile)

About Facultad de Medicina y Facultad de Ingenieria y Ciencias Aplicadas, Universidad de los Andes, San Carlos de Apoquindo, 2200 Santiago, Chile

, 
Reetz Manfred T.

About Reetz Manfred T.

(Max-Planck-Institut fuer Kohlenforschung, 45070 Muelheim, Germany)

About Max-Planck-Institut fuer Kohlenforschung, 45070 Muelheim, Germany

, 
Reetz Manfred T.

About Reetz Manfred T.

(Chemistry Department, Philipps-University, 35032 Marburg, Germany)

About Chemistry Department, Philipps-University, 35032 Marburg, Germany

, 
Asenjo Juan A.

About Asenjo Juan A.

(Centre for Biotechnology and Bioengineering, CeBiB, Department of Chemical Engineering and Biotechnology, University of Chile, Beauchef, 851 Santiago, Chile)

About Centre for Biotechnology and Bioengineering, CeBiB, Department of Chemical Engineering and Biotechnology, University of Chile, Beauchef, 851 Santiago, Chile

, 
Parra Loreto P.

About Parra Loreto P.

(Institute for Biological and Medical Engineering, Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Catolica de Chile, Avenida Vicuna Mackenna, 4860 Santiago, Chile)

About Institute for Biological and Medical Engineering, Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Catolica de Chile, Avenida Vicuna Mackenna, 4860 Santiago, Chile

, 
Parra Loreto P.

About Parra Loreto P.

(Department of Chemical and Bioprocesses Engineering, School of Engineering, Pontificia Universidad Catolica de Chile, Avenida Vicuna Mackenna, 4860 Santiago, Chile)

About Department of Chemical and Bioprocesses Engineering, School of Engineering, Pontificia Universidad Catolica de Chile, Avenida Vicuna Mackenna, 4860 Santiago, Chile


Material：
Enzyme and Microbial Technology  (EMT)

About Enzyme and Microbial Technology


Volume： 100  Page： 60-70  Publication year： 2017 
JST Material Number： A0989B  ISSN： 0141-0229  Document type： Article
Article type： 原著論文  Country of issue： United States (USA)  Language： ENGLISH (EN)
Abstract/Point：
Abstract/Point
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Enzymes active at low temperat...
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Thesaurus term：
Thesaurus term/Semi thesaurus term
Keywords indexed to the article.
All keywords is available on JDreamIII(charged).
On J-GLOBAL, this item will be available after more than half a year after the record posted. In addtion, medical articles require to login to MyJ-GLOBAL.
*xylanase

About xylanase

, 
protein engineering

About protein engineering

, 
characterization

About characterization

, 
enzyme

About enzyme

, 
clone

About clone

, 
enzyme activity

About enzyme activity

, 
*thermal stability

About thermal stability

, 
biocatalyst

About biocatalyst

, 
*low temperature

About low temperature

, 
stereoselectivity

About stereoselectivity

, 
*mutagenesis

About mutagenesis


Semi thesaurus term：
Thesaurus term/Semi thesaurus term
Keywords indexed to the article.
All keywords is available on JDreamIII(charged).
On J-GLOBAL, this item will be available after more than half a year after the record posted. In addtion, medical articles require to login to MyJ-GLOBAL.
分子動力学シミュレーション

About 分子動力学シミュレーション

, 
定向進化

About 定向進化

, 
特異性

About 特異性

, 
*飽和変異誘発

About 飽和変異誘発

, 【Automatic Indexing@JST】
Author keywords (5)：
Xylanase

About Xylanase

, 
Cold-active enzymes

About Cold-active enzymes

, 
Thermostability

About Thermostability

, 
Directed evolution

About Directed evolution

, 
epPCR

About epPCR

JST classification (1)：
JST classification
Category name(code) classified by JST.
Enzyme in general 
(EB09010D)

About Enzyme in general

Terms in the title (8)：
Terms in the title
Keywords automatically extracted from the title.
低温

About 低温

, 
活性

About 活性

, 
キシラナーゼ

About キシラナーゼ

, 
熱安定性

About 熱安定性

, 
進化

About 進化

, 
段階

About 段階

, 
集束

About 集束

, 
飽和変異誘発

About 飽和変異誘発


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