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J-GLOBAL ID：201702265557109118   Reference number：17A0014478

Development of a versatile method for targeted gene deletion and insertion by using the pyrF gene in the psychrotrophic bacterium, Shewanella livingstonensis Ac10

低温細菌Shewanella livingstonensis Ac10におけるpyrF遺伝子を用いた標的遺伝子の欠失と挿入のための汎用的な方法の開発

Author (4)： Ito Tomokazu (Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan) ,  Gong Chunjie (Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan) ,  Kawamoto Jun (Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan) ,  Kurihara Tatsuo (Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan)
Material： Journal of Bioscience and Bioengineering
Volume： 122  Issue： 6  Page： 645-651  Publication year： Dec. 2016 
JST Material Number： G0535B  ISSN： 1389-1723  CODEN： JBBIF6  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Thesaurus term： psychrotroph ,...
Semi thesaurus term： *Shewanella livingstonensis Ac10 ,...

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Author keywords (5)： Key words Gene deletion ,  Gene insertion ,  Homologous recombination ,  pyrF ,  Shewanella

JST classification (2)： Gene manipulation  (EC02050B) ,  Microorganism biochemistry  (EG03020N)

Substance index (1)： Eicosapentaenoic Acid

Reference (21)：

• Hau, H. H. and Gralnick, J. A.: Ecology and biotechnology of the genus Shewanella, Annu. Rev. Microbiol., 61, 237-258 (2007).
• Richter, K., Schicldberger, M., and Gescher, J.: Dissimilatory reduction of extracellular electron acceptors in anaerobic respiration, Appl. Environ. Microbiol., 78, 913-921 (2012).
• Kulakova, L, Galkin, A., Kurihara, T., Yoshimura, T., and Esaki, N.: Cold-active serine alkaline protease from the psychrotrophic bacterium Shewanella strain Ac10: gene cloning and enzyme purification and characterization, Appl. Environ. Microbiol., 65, 611-617 (1999).
• Yoshimune, K., Galkin, A., Kulakova, L, Yoshimura, T., and Esaki, N.: Cold-active DnaK of an Antarctic psychrotroph Shewanella sp. Ac10 supporting the growth of dnaK-null mutant of Escherichia coli at cold temperatures, Extremophiles, 9, 145-150 (2005).
• Kawamoto, J., Kurihara, T., Kitagawa, M., Kato, L, and Esaki, N.: Proteomic studies of an Antarctic cold-adapted bacterium, Shewanella livingstonensis Ac10, for global identification of cold-inducible proteins, Extremophiles, 11, 819-826 (2007).

Terms in the title (7)： 低温細菌 ,  Shewanella ,  遺伝子 ,  標的遺伝子 ,  欠失 ,  汎用 ,  開発