Art

J-GLOBAL ID：201702269547538170   Reference number：17A0418331

RNAi-mediated knockdown of mouse melanocortin-4 receptor in vitro and in vivo, using an siRNA expression construct based on the mir-187 precursor

mir-187前駆体を基にしたsiRNA発現コンストラクトを用いいる,in vitroおよびin vivoでのマウスメラノコルチン-4受容体のRNAi介在性ノックダウン

Author (14)： Kato Minoru (Research Unit/Neuroscience, Mitsubishi Tanabe Pharma Corporation, 1000 Kamoshida, Aoba-ku, Yokohama, Kanagawa 227-0033, Japan) ,  Huang Yi-Ying (Laboratory of Chronogenomics, Mitsubishi Kagaku Institute of Life Science, 11 Minamiooya, Machida, Tokyo 194-8511, Japan) ,  Matsuo Mina (Experimental Animal Laboratory, Mitsubishi Kagaku Institute of Life Science, 11 Minamiooya, Machida, Tokyo 194-8511, Japan) ,  Matsuo Mina (Present address: Division of Animal Experimental Laboratory, Life Science Research Center, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan) ,  Takashina Yoko (Pharmacology Laboratory, Mitsubishi Tanabe Pharma Corporation, 1000 Kamoshida, Aoba-ku, Yokohama, Kanagawa 227-0033, Japan) ,  Sasaki Kazuyo (Pharmacology Laboratory, Mitsubishi Tanabe Pharma Corporation, 1000 Kamoshida, Aoba-ku, Yokohama, Kanagawa 227-0033, Japan) ,  Horai Yasushi (Safety Research Laboratory, Mitsubishi Tanabe Pharma Corporation, 1000 Kamoshida, Aoba-ku, Yokohama, Kanagawa 227-0033, Japan) ,  Juni Aya (Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan) ,  Kamijo Shin-Ichi (Experimental Animal Laboratory, Mitsubishi Kagaku Institute of Life Science, 11 Minamiooya, Machida, Tokyo 194-8511, Japan) ,  Kamijo Shin-Ichi (Present address: Division of Planning of Laboratory Animal Facility, Lifewill Corporation, 2740-12 Shimo-oyamada, Machida, Tokyo 194-0202, Japan) ,  Saigo Kaoru (Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan) ,  Ui-Tei Kumiko (Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan) ,  Tei Hajime (Present address: Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa, Ishikawa 920-1192, Japan) ,  Tei Hajime (Laboratory of Chronogenomics, Mitsubishi Kagaku Institute of Life Science, 11 Minamiooya, Machida, Tokyo 194-8511, Japan)
Material： Experimental Animals  (実験動物ニュース)
Volume： 66  Issue： 1  Page： 41-50(J-STAGE)  Publication year： 2017 
JST Material Number： Z0755A  ISSN： 1341-1357  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： antisense RNA ,...
Semi thesaurus term： miR-187 ,...

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Author keywords (4)： mir-187 ,  mMC4R ,  RNAi ,  Transgenic mice

JST classification (5)： Research methods in genetics  (EC01020N) ,  Gene manipulation  (EC02050B) ,  Gene expression  (EC02040Q) ,  Receptors on cell membrane  (EF04030C) ,  Central nervous system  (EJ12030F)

Reference (35)：

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Terms in the title (8)： 前駆体 ,  siRNA ,  発現 ,  マウス ,  メラノコルチン ,  受容体 ,  RNAi ,  ノックダウン