Art

J-GLOBAL ID：201702273367176110   Reference number：17A0980100

Enhanced mRNA-protein fusion efficiency of a single-domain antibody by selection of mRNA display with additional random sequences in the terminal translated regions

末端翻訳領域の追加的ランダム配列を伴うmRNAディスプレイの選択による単一ドメイン抗体のmRNA-タンパク質融合効率の増強

Author (5)： Takahashi Kazuki (Graduate School of Science and Engineering, Saitama University) ,  Sunohara Masato (Graduate School of Science and Engineering, Saitama University) ,  Terai Takuya (Graduate School of Science and Engineering, Saitama University) ,  Kumachi Shigefumi (Graduate School of Science and Engineering, Saitama University) ,  Nemoto Naoto (Graduate School of Science and Engineering, Saitama University)
Material： Biophysics and Physicobiology (Web)  (Biophys Physicobiology (Web))
Volume： 14  Page： 23-28(J-STAGE)  Publication year： 2017 
JST Material Number： U0030A  ISSN： 2189-4779  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： gene ,...
Semi thesaurus term： *coding region ,...

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Author keywords (4)： Nanobody ,  variable domain of heavy chain of heavy chain antibody ,  electrophoresis mobility shift assay ,  in vitro translation

JST classification (2)： Production and application of antigens,antibodies,complements  (ED04030W) ,  Biophysical research methods  (EA03020V)

Reference (21)：

• [1] Josephson, K., Ricardo, A. & Szostak, J. W. mRNA display: from basic principles to macrocycle drug discovery. Drug Discov. Today 19, 388-399 (2014).
• [2] Valencia, C. A., Zou, J. & Liu, R. In vitro selection of proteins with desired characteristics using mRNA-display. Methods 60, 55-69 (2013).
• [3] Nemoto, N., Miyamoto-Sato, E., Husimi, Y. & Yanagawa, H. In vitro virus: bonding of mRNA bearing puromycin at the 3′-terminal end to the C-terminal end of its encoded protein on the ribosome in vitro. FEBS Lett. 414, 405-408 (1997).
• [4] Roberts, R. W. & Szostak, J. W. RNA-peptide fusions for the in vitro selection of peptides and proteins. Proc. Natl. Acad. Sci. USA 94, 12297-12302 (1997).
• [5] Tabata, N., Sakuma, Y., Honda, Y., Doi, N., Takashima, H., Miyamoto-Sato, E., et al. Rapid antibody selection by mRNA display on a microfluidic chip. Nucleic Acids Res. 37, e64 (2009).

Terms in the title (7)： 翻訳領域 ,  mRNAディスプレイ ,  単一ドメイン抗体 ,  mRNA ,  タンパク質 ,  融合 ,  増強