Research keywords (3):
G-quadruplex
, DNA methylation
, Biosensing
Research theme for competitive and other funds (5):
2021 - 2025 等温PCRを用いた修飾塩基測定法の開発と在宅がん診断への展開
2017 - 2020 Development of a methylated DNA sensing system for circulating tumor cells detection
2015 - 2017 Identification of ribozyme catalyzes DNA methylation to develop gene specific DNA methylation system
2012 - 2014 Identification of methylated DNA-binding RNA aptamer and development of high sensitive DNA methylation detection system
2008 - 2010 エピジェネティクス解析のための新規アプタマーの探索と応用
Papers (66):
Marika Fujita, Masanori Goto, Masayoshi Tanaka, Wataru Yoshida. Detection of CpG methylation level using methyl-CpG-binding domain-fused fluorescent protein. Analytical methods : advancing methods and applications. 2023. 15. 19. 2294-2299
Ayano Goto, Wataru Yoshida. Hybridization-based CpG methylation level detection using methyl-CpG-binding domain-fused luciferase. Analytical and bioanalytical chemistry. 2023
Takamichi Miyata, Hazuki Shimamura, Ryutaro Asano, Wataru Yoshida. Universal Design of Luciferase Fusion Proteins for Epigenetic Modifications Detection Based on Bioluminescence Resonance Energy Transfer. Analytical Chemistry. 2023. 95. 7. 3799-3805
Masanori Goto, Yuji Baba, Wataru Yoshida. Quantitative detection of CpG methylation level on G-quadruplex and i-motif-forming DNA by recombinase polymerase amplification. Analytical and Bioanalytical Chemistry. 2022. 414. 20. 6223-6231
Natsumi Taka, Shoya Asami, Mikiya Sakamoto, Toru Matsui, Wataru Yoshida. Quantification of Global DNA Hydroxymethylation Level Using UHRF2 SRA-Luciferase Based on Bioluminescence Resonance Energy Transfer. Analytical Chemistry. 2022. 94. 24. 8618-8624
Saito Taiki, Yoshida Wataru, Yokoyama Tomomi, Ikebukuro Kazunori. 2P-234 Aptamer selection focusing on G-quadruplex forming sequence in genome. 2013. 65. 163-163
Hiraoka Daisuke, Yoshida Wataru, Hata Kenichiro, Ikebukuro Kazunori. 2Ep02 Development of simple DNA methylation level evaluating method using magnetic beads. 2011. 63. 144-144
HIRAOKA Daisuke, MURAKAMI Yoshiyuki, YOSHIDA Wataru, HATA Kenichiro, IKEBUKURO Kazunori. 3P-1081 Novel method for evaluation of DNA methylation level using methyl CpG binding domain. 2010. 22. 78-78
YOSHIDA Wataru, SODE Koji, IKEBUKURO Kazunori. 1J11-2 Homogeneous IgE sensing using aptameric enzyme subunit. 2007. 19. 209-209