Research theme for competitive and other funds (5):
2022 - 2025 Optimization of Neutron Reflectometry for analysis of biological membrane
2021 - 2024 Optimization of multiple method baced on neutron reflectometry for analysis of biogenesis of Gram-negative bacterial membrane.
2019 - 2021 Elucidation of the molecular mechanism of E. coli BAM complex by in vitro assay system.
2018 - 2021 Elucidation of the process of membrane protein insrtion and folding.
2018 - 2020 Analysis of the assembly of the beta-barrel membrane proteins into the E. coli outer membrane by the EMM assembly assay
Papers (23):
Nakajohn Thewasano, Edward M Germany, Yuki Maruno, Yukari Nakajima, Takuya Shiota. Categorization of Escherichia coli Outer Membrane Proteins by Dependence on Accessory Proteins of the β-barrel Assembly Machinery Complex. The Journal of biological chemistry. 2023. 104821-104821
Yuko Ikegawa, Christophe Combet, Mathieu Groussin, Vincent Navratil, Sabrina Safar-Remali, Takuya Shiota, Abdel Aouacheria, Sa Kan Yoo. Evidence for existence of an apoptosis-inducing BH3-only protein, sayonara, in Drosophila. The EMBO journal. 2023. 42. 8. e110454
Xiaoyu Chen, Yue Ding, Rebecca S Bamert, Anton P Le Brun, Anthony P Duff, Chun-Ming Wu, Hsien-Yi Hsu, Takuya Shiota, Trevor Lithgow, Hsin-Hui Shen. Substrate-dependent arrangements of the subunits of the BAM complex determined by neutron reflectometry. Biochimica et biophysica acta. Biomembranes. 2021. 1863. 9. 183587-183587
Shiota T. [Molecular architecture and function of mitochondrial protein entry gate]. Seikagaku. The Journal of Japanese Biochemical Society. 2017. 89. 2. 282-285