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J-GLOBAL ID：201802228078469498   Reference number：18A1135674

Phosphorylated and non-phosphorylated HCK kinase domains produced by cell-free protein expression

無細胞蛋白質発現により産生されるリン酸化および非リン酸化Hckキナーゼドメイン【JST・京大機械翻訳】

Author (15)： Katsura Kazushige (Protein Functional and Structural Biology Team, RIKEN Center for Life Science Technology, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan) ,  Tomabechi Yuri (Protein Functional and Structural Biology Team, RIKEN Center for Life Science Technology, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan) ,  Matsuda Takayoshi (Protein Functional and Structural Biology Team, RIKEN Center for Life Science Technology, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan) ,  Yonemochi Mayumi (Protein Functional and Structural Biology Team, RIKEN Center for Life Science Technology, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan) ,  Yonemochi Mayumi (Drug Discovery Structural Biology Platform Unit, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan) ,  Mikuni Junko (Protein Functional and Structural Biology Team, RIKEN Center for Life Science Technology, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan) ,  Mikuni Junko (Drug Discovery Structural Biology Platform Unit, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan) ,  Ohsawa Noboru (Protein Functional and Structural Biology Team, RIKEN Center for Life Science Technology, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan) ,  Terada Takaho (RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan) ,  Yokoyama Shigeyuki (RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan) ,  Kukimoto-Niino Mutsuko (Protein Functional and Structural Biology Team, RIKEN Center for Life Science Technology, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan) ,  Kukimoto-Niino Mutsuko (Drug Discovery Structural Biology Platform Unit, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan) ,  Takemoto Chie (Protein Functional and Structural Biology Team, RIKEN Center for Life Science Technology, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan) ,  Shirouzu Mikako (Protein Functional and Structural Biology Team, RIKEN Center for Life Science Technology, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan) ,  Shirouzu Mikako (Drug Discovery Structural Biology Platform Unit, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan)
Material： Protein Expression and Purification  (Protein Expression & Purification)
Volume： 150  Page： 92-99  Publication year： 2018 
JST Material Number： W0282A  ISSN： 1046-5928  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Abstract/Point： Since phosphorylation is invol...

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Thesaurus term： intermolecular interaction ,  peptide ,  substrate (biochemistry) ,  protein kinase ,  complex (substance) ,  Escherichia coli ,  gene ,  plasmid ,  chaperone protein ,  *phosphorylation ,  solubility (ratio) ,  human ,  protein synthesis ,  *kinase ,  microorganism
Semi thesaurus term： 阻害剤 , 【Automatic Indexing@JST】

Author keywords (6)： Hematopoietic cell kinase (HCK) ,  X-ray crystallography ,  Cell-free protein synthesis ,  Solubility ,  Chaperone ,  Autophosphorylation

JST classification (2)： Enzyme in general  (EB09010D) ,  Gene expression  (EC02040Q)

Terms in the title (6)： 無細胞蛋白質 ,  発現 ,  リン酸化 ,  Hck ,  キナーゼ ,  ドメイン