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J-GLOBAL ID：201802228520462530   Reference number：18A1063303

Real-time observation of flexible domain movements in CRISPR-Cas9

CRISPR-Cas9における柔軟領域運動の実時間観測【JST・京大機械翻訳】

Author (8)： Osuka Saki (Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan) ,  Isomura Kazushi (Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan) ,  Kajimoto Shohei (Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan) ,  Komori Tomotaka (Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan) ,  Nishimasu Hiroshi (Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan) ,  Shima Tomohiro (Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan) ,  Nureki Osamu (Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan) ,  Uemura Sotaro (Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan)
Material： EMBO Journal  (EMBO J)
Volume： 37  Issue： 10  Page： e96941  Publication year： 2018 
JST Material Number： A0911B  ISSN： 0261-4189  CODEN： EMJODG  Document type： Article
Article type： 原著論文  Country of issue： United States (USA)  Language： ENGLISH (EN)

Abstract/Point： The CRISPR-associated protein ...

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Thesaurus term： fluctuation ,  multiplex ,  flexibility ,  crystal structure ,  steric conformation ,  catalyst ,  *DNA ,  plasticity
Semi thesaurus term： DNA切断 ,  動的変化 ,  単一分子 ,  ドメイン構造 ,  sgRNA ,  実時間 ,  *Cas9 , 【Automatic Indexing@JST】

Author keywords (3)： conformational plasticity ,  gene editing ,  intramolecular FRET

JST classification (3)： Molecular structure  (EB03020Y) ,  Molecular genetics in general  (EC02010J) ,  Enzyme in general  (EB09010D)

Terms in the title (4)： CRISPR-Cas9 ,  運動 ,  実時間 ,  観測