Art

J-GLOBAL ID：201802239544996256   Reference number：18A0769468

Flow cytometry-based method for rapid and high-throughput screening of hybridoma cells secreting monoclonal antibody

モノクローナル抗体を分泌するハイブリドーマ細胞の迅速かつハイスループットスクリーニングのためのフローサイトメトリーに基づく方法

Author (4)： Akagi Sachi (Laboratory of Molecular Biology, Graduate School of Engineering, Yokohama National University, Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan) ,  Nakajima Chika (Laboratory of Molecular Biology, College of Engineering Sciences, Yokohama National University, Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan) ,  Tanaka Yoichiro (Instrumental Analysis Center, Yokohama National University, Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan) ,  Kurihara Yasuyuki (Laboratory of Molecular Biology, Graduate School of Engineering, Yokohama National University, Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan)
Material： Journal of Bioscience and Bioengineering
Volume： 125  Issue： 4  Page： 464-469  Publication year： Apr. 2018 
JST Material Number： G0535B  ISSN： 1389-1723  CODEN： JBBIF6  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Thesaurus term： *monoclonal antibody ,...
Semi thesaurus term： immunoassay ,...

Register(free) or Log In to access     To see more with JDream III (charged).   {{ this.onShowAbsJLink("http://jdream3.com/lp/jglobal/index.html?docNo=18A0769468&from=J-GLOBAL&jstjournalNo=G0535B") }}

Author keywords (5)： Monoclonal antibody ,  Flow cytometry ,  Hybridoma ,  Myeloma ,  Peptide antigen

JST classification (1)： Production and application of antigens,antibodies,complements  (ED04030W)

Reference (11)：

• Köhler, G. and Milstein, C.: Continuous cultures of fused cells secreting anti-body of predefined specificity, Nature, 256, 495-497 (1975).
• Ecker, D. M., Jones, S. D., and Levine, H. L.: The therapeutic monoclonal antibody market, MAbs, 7, 9-14 (2015).
• Hoogenboom, H. R.: Selecting and screening recombinant antibody libraries, Nat. Biotechnol., 23, 1105-1116 (2005).
• Yoshikawa, K., Okazaki, I. M., Eto, T., Kinoshita, K., Muramatsu, M., Nagaoka, H., and Honjo, T.: AID enzyme-induced hypermutation in an actively transcribed gene in fibroblasts, Science, 296, 2033-2036 (2002).
• Chen, S., Qiu, J., Chen, C., Liu, C., Liu, Y., An, L., Jia, J., Tang, J., Wu, L., and Hang, H.: Affinity maturation of anti-TNF-alpha scFv with somatic hypermutation in non-B cells, Protein Cell, 3, 460-469 (2012).

Terms in the title (5)： モノクローナル抗体 ,  分泌 ,  ハイブリドーマ細胞 ,  ハイスループットスクリーニング ,  フローサイトメトリー