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J-GLOBAL ID：201802239560015105   Reference number：18A0318537

Validation of Anti-CSPα, SNAP25, Tyrosine Hydroxylase, Ubiquitin, Cleaved Caspase 3, and pSer PKC Motif Antibodies for Utilization in Western Blotting

ウエスタンブロッティングでの利用のための抗CSPα,SNAP25,チロシンヒドロキシラーゼ,ユビキチン,Cleaved Caspase3,およびpSer PKCモチーフ抗体の検証

Author (6)： Shirafuji Toshihiko (Department of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hiroshima University) ,  Ueyama Takehiko (Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University) ,  Tanaka Shigeru (Department of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hiroshima University) ,  Hide Izumi (Department of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hiroshima University) ,  Saito Naoaki (Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University) ,  Sakai Norio (Department of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hiroshima University)
Material： Acta Histochemica et Cytochemica (Web)  (Acta Histochem Cytochem (Web))
Volume： 50  Issue： 6  Page： 177-180(J-STAGE)  Publication year： 2017 
JST Material Number： U0129A  ISSN： 1347-5800  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： cultured cell ,...
Semi thesaurus term： 抗CSPα抗体 ,...

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Author keywords (3)： validation ,  antibody ,  Western blotting

JST classification (2)： Bioassay  (EA03060N) ,  Production and application of antigens,antibodies,complements  (ED04030W)

Reference (4)：

• 1 Couchman, J. R. (2009) Commercial antibodies: the good, bad, and really ugly. J. Histochem. Cytochem. 57; 7-8.
• 2 Hewitt, S. M., Baskin, D. G., Frevert, C. W., Stahl, W. L. and Rosa-Molinar, E. (2014) Controls for immunohistochemistry: the Histochemical Society’s standards of practice for validation of immunohistochemical assays. J. Histochem. Cytochem. 62; 693-7.
• 3 Pradidarcheep, W., Labruyere, W. T., Dabhoiwala, N. F. and Lamers, W. H. (2008) Lack of specificity of commercially available antisera: better specifications needed. J. Histochem. Cytochem. 56; 1099-111.
• 4 Rhodes, K. J. and Trimmer, J. S. (2006) Antibodies as valuable neuroscience research tools versus reagents of mass distraction. J. Neurosci. 26; 8017-20.

Terms in the title (9)： ウエスタンブロッティング ,  CSP ,  SNAP25 ,  チロシンヒドロキシラーゼ ,  ユビキチン ,  PKC ,  モチーフ ,  抗体 ,  検証