Art

J-GLOBAL ID：201802246135198750   Reference number：18A0630270

High sensitive RNA detection by one-step RT-PCR using the genetically engineered variant of DNA polymerase with reverse transcriptase activity from hyperthermophilies

超好熱菌由来の逆転写酵素活性を持つDNAポリメラーゼの遺伝子操作変異体を用いたワンステップRT-PCR法による高感度RNA検出

Author (9)： Okano Hiroyuki (Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kitashirakawa Sakyo-ku, Kyoto 606-8502, Japan) ,  Baba Misato (Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kitashirakawa Sakyo-ku, Kyoto 606-8502, Japan) ,  Kawato Katsuhiro (Department of Bioscience, School of Science and Technology, Kwansei-Gakuin University, 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan) ,  Hidese Ryota (Department of Bioscience, School of Science and Technology, Kwansei-Gakuin University, 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan) ,  Yanagihara Itaru (Department of Developmental Medicine, Research Institute, Osaka Women’s and Children’s Hospital, 840 Murodo-cho, Izumi 594-1101, Osaka, Japan) ,  Kojima Kenji (Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kitashirakawa Sakyo-ku, Kyoto 606-8502, Japan) ,  Takita Teisuke (Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kitashirakawa Sakyo-ku, Kyoto 606-8502, Japan) ,  Fujiwara Shinsuke (Department of Bioscience, School of Science and Technology, Kwansei-Gakuin University, 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan) ,  Yasukawa Kiyoshi (Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kitashirakawa Sakyo-ku, Kyoto 606-8502, Japan)
Material： Journal of Bioscience and Bioengineering
Volume： 125  Issue： 3  Page： 275-281  Publication year： Mar. 2018 
JST Material Number： G0535B  ISSN： 1389-1723  CODEN： JBBIF6  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Thesaurus term： DNA ,...
Semi thesaurus term： Thermococcus kodakarensis ,...

Register(free) or Log In to access     To see more with JDream III (charged).   {{ this.onShowAbsJLink("http://jdream3.com/lp/jglobal/index.html?docNo=18A0630270&from=J-GLOBAL&jstjournalNo=G0535B") }}

Author keywords (5)： cDNA synthesis ,  DNA polymerase ,  One-step RT-PCR ,  PCR ,  Reverse transcriptase

JST classification (3)： Research methods in genetics  (EC01020N) ,  Applications of enzymes  (EB09100B) ,  Microorganism biochemistry  (EG03020N)

Reference (24)：

• Gerard, G. F., Potter, R. J., Smith, M. D., Rosenthal, K., Dhariwal, G., Lee, J., and Chatterjee, D. K.: The role of template-primer in protection of reverse transcriptase from thermal inactivation, Nucleic Acids Res., 30, 3118-3129 (2002).
• Arezi, B. and Hogrefe, H.: Novel mutations in Moloney murine leukemia virus reverse transcriptase increase thermostability through tighter binding to template-primer, Nucleic Acids Res., 37, 473-481 (2009).
• Yasukawa, K., Mizuno, M., Konishi, A., and Inouye, K.: Increase in thermal stability of Moloney murine leukaemia virus reverse transcriptase by site-directed mutagenesis, J. Biotechnol., 150, 299-306 (2010).
• Baranauskas, A., Paliksa, S., Alzbutas, G., Vaitkevicius, M., Lubiene, J., Letukiene, V., Burinskas, S., Sasnauskas, G., and Skirgaila, R.: Generation and characterization of new highly thermostable and processive M-MuLV reverse transcriptase variants, Protein Eng. Des. Sel., 25, 657-668 (2012).
• Konishi, A., Ma, X., and Yasukawa, K.: Stabilization of Moloney murine leukemia virus reverse transcriptase by site-directed mutagenesis of the surface residue Val433, Biosci. Biotechnol. Biochem., 78, 147-150 (2014).

Terms in the title (9)： 好熱菌 ,  転写 ,  酵素活性 ,  DNAポリメラーゼ ,  遺伝子操作 ,  変異体 ,  RT-PCR法 ,  高感度 ,  RNA