Art

J-GLOBAL ID：201802246844333047   Reference number：18A1031130

Optimisation of methods for bacterial skin microbiome investigation: primer selection and comparison of the 454 versus MiSeq platform

細菌皮膚ミクロビオーム研究のための方法の最適化: 454対MISEQプラットフォームのプライマー選択と比較【JST・京大機械翻訳】

Author (9)： Castelino Madhura (NIHR Manchester Musculoskeletal Biomedical research Unit, Central Manchester Foundation Trust, Manchester Academic Health Sciences Centre, Manchester, England) ,  Eyre Stephen (Arthritis Research UK Centre for Genetics and Genomics, Centre for Musculoskeletal Research, Institute of Inflammation and Repair, University of Manchester, Manchester, UK) ,  Moat John (Microbiology and Virology Unit, Institute of Inflammation and Repair, The University of Manchester, Manchester, UK) ,  Fox Graeme (DNA Sequencing Facility, Faculty of Medical & Human Sciences, The University of Manchester, Manchester, UK) ,  Martin Paul (Arthritis Research UK Centre for Genetics and Genomics, Centre for Musculoskeletal Research, Institute of Inflammation and Repair, University of Manchester, Manchester, UK) ,  Ho Pauline (NIHR Manchester Musculoskeletal Biomedical research Unit, Central Manchester Foundation Trust, Manchester Academic Health Sciences Centre, Manchester, England) ,  Upton Mathew (School of Biomedical and Healthcare Sciences, Plymouth University Peninsular Schools of Medicine and Dentistry, Plymouth, UK) ,  Barton Anne (NIHR Manchester Musculoskeletal Biomedical research Unit, Central Manchester Foundation Trust, Manchester Academic Health Sciences Centre, Manchester, England) ,  Barton Anne (Arthritis Research UK Centre for Genetics and Genomics, Centre for Musculoskeletal Research, Institute of Inflammation and Repair, University of Manchester, Manchester, UK)
Material： BMC Microbiology (Web)
Volume： 17  Issue： 1  Page： 23  Publication year： 2017 
JST Material Number： U7367A  ISSN： 1471-2180  Document type： Article
Article type： 原著論文  Country of issue： United Kingdom (GBR)  Language： ENGLISH (EN)

Abstract/Point： Background The composition of ...

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Thesaurus term： voluntary workers ,  Biomass ,  Microbiome ,  human ,  protocol ,  DNA ,  *Bacteria ,  rRNA ,  robustness ,  rRNA gene ,  *optimization ,  hypervariable region ,  16S rRNA gene ,  16S rRNA ,  *microorganism ,  *modification
Semi thesaurus term： *常在菌叢ゲノム , 【Automatic Indexing@JST】

Author keywords (9)： Skin microbiome ,  Bacterial microbiome ,  Low biomass ,  16s rRNA gene ,  Miseq platform ,  Next generation sequencing ,  Methods ,  Optimisation ,  Primer selection

JST classification (2)： Research methods in genetics  (EC01020N) ,  Microorganism test methods  (EG02020G)

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Terms in the title (7)： 細菌 ,  皮膚 ,  ミクロビオーム ,  研究 ,  最適化 ,  プラットフォーム ,  プライマー