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J-GLOBAL ID：201802247631982471   Reference number：18A1607744

Application of chromosomal gene insertion into Escherichia coli for expression of recombinant proteins

大腸菌Escherichia coliの染色体への遺伝子挿入を利用した組換蛋白質の発現

Author (7)： Nakamura Tsutomu (National Institute of Advanced Industrial Science and Technology, 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan) ,  Koma Daisuke (Osaka Research Institute of Industrial Science and Technology, 1-6-50 Morinomiya, Joto-ku, Osaka 536-8553, Japan) ,  Oshima Maki (National Institute of Advanced Industrial Science and Technology, 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan) ,  Hoshino Hideto (National Institute of Advanced Industrial Science and Technology, 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan) ,  Ohmoto Takashi (Osaka Research Institute of Industrial Science and Technology, 1-6-50 Morinomiya, Joto-ku, Osaka 536-8553, Japan) ,  Uegaki Koichi (National Institute of Advanced Industrial Science and Technology, 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan) ,  Uegaki Koichi (Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara 631-8505, Japan)
Material： Journal of Bioscience and Bioengineering
Volume： 126  Issue： 2  Page： 266-272  Publication year： Aug. 2018 
JST Material Number： G0535B  ISSN： 1389-1723  CODEN： JBBIF6  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Thesaurus term： gene manipulation ,...
Semi thesaurus term： d-アミノアシラーゼ ,...

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Author keywords (5)： Chromosomal gene insertion ,  d-Aminoacylase ,  Multiple insertions ,  Red-mediated recombination system ,  Single insertion

JST classification (3)： Gene manipulation  (EC02050B) ,  Substance production by microorganisms,cultured tissues/cells in general  (FK03010P) ,  Molecular structure  (EB03020Y)

Reference (18)：

• Balzer, S., Kucharova, V., Megerle, J., Lale, R., Brautaset, T., and Valla, S.: A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli, Microb. Cell Fact., 12, 26 (2013).
• Terpe, K.: Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems, Appl. Microbiol. Biotechnol., 72, 211-222 (2006).
• Rosano, G. L. and Ceccarelli, E. A.: Recombinant protein expression in microbial systems, Front. Microbiol., 5, 341 (2014).
• Singh, A., Upadhyay, V., Upadhyay, A. K., Singh, S. M., and Panda, A. K.: Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process, Microb. Cell Fact., 14, 41 (2015).
• Hannig, G. and Makrides, S. C.: Strategies for optimizing heterologous protein expression in Escherichia coli, Trends Biotechnol., 16, 54-60 (1998).

Terms in the title (6)： 大腸菌 ,  Escherichia ,  染色体 ,  遺伝子挿入 ,  組換蛋白質 ,  発現