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J-GLOBAL ID：201802270736443874   Reference number：18A1998295

Disulfide bonds elimination of endoglucanase II from Trichoderma reesei by site-directed mutagenesis to improve enzyme activity and thermal stability: An experimental and theoretical approach

酵素活性および熱安定性を改善するための部位特異的変異誘発によるTrichoderma reesei由来エンドグルカナーゼIIのジスルフィド結合脱離:実験的および理論的アプローチ【JST・京大機械翻訳】

Author (11)： Akbarzadeh Ali (Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran) ,  Akbarzadeh Ali (Gene Transfer Pioneers Research Group, Shahid Beheshti University of Medical Science, Tehran, Iran) ,  Akbarzadeh Ali (Biotechnology Protein Research Center (PRC), Shahid Beheshti University, GC, Tehran, Iran) ,  Pourzardosht Navid (Biochemistry Department, Guilan University of Medical Sciences, Rasht, Iran) ,  Dehnavi Ehsan (Gene Transfer Pioneers Research Group, Shahid Beheshti University of Medical Science, Tehran, Iran) ,  Ranaei Siadat Seyed Omid (Biotechnology Protein Research Center (PRC), Shahid Beheshti University, GC, Tehran, Iran) ,  Zamani Mohammad Reza (Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran) ,  Motallebi Mostafa (Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran) ,  Nikzad Jamnani Farnaz (Biotechnology Protein Research Center (PRC), Shahid Beheshti University, GC, Tehran, Iran) ,  Aghaeepoor Mojtaba (Gene Transfer Pioneers Research Group, Shahid Beheshti University of Medical Science, Tehran, Iran) ,  Barshan Tashnizi Mohammad (Department of Life Science Engineering, Faculty of New Science and Technologies, University of Tehran, Tehran, Iran)
Material： International Journal of Biological Macromolecules  (Int J Biol Macromol)
Volume： 120  Issue： PB  Page： 1572-1580  Publication year： 2018 
JST Material Number： T0898A  ISSN： 0141-8130  CODEN： IJBMDR  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Abstract/Point： EndoglucanaseII (Cel5A) of Tri...

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Thesaurus term： *cellulase ,  flexibility ,  complex(substance) ,  *disulfide bond ,  substrate(biochemistry) ,  *desorption ,  *enzyme ,  *Trichoderma reesei ,  *thermal stability ,  *site-directed mutagenesis ,  thermophilic ,  *enzyme activity ,  amino acid ,  nitrogen heterocyclic compound ,  primary amine
Semi thesaurus term： Pichia pastoris ,  触媒効率 , 【Automatic Indexing@JST】

Author keywords (4)： Endoglucanase II ,  Site directed mutagenesis ,  Disulfide bond ,  Thermal stability

JST classification (1)： Enzyme in general  (EB09010D)

Substance index (1)： Histidine

Terms in the title (9)： 酵素活性 ,  熱安定性 ,  部位特異的変異誘発 ,  Trichoderma ,  エンドグルカナーゼ ,  ジスルフィド結合 ,  脱離 ,  実験 ,  理論