Art

J-GLOBAL ID：201802287388433901   Reference number：18A0061607

A Single-Step “Breeding” Generated a Diagnostic Anti-cortisol Antibody Fragment with Over 30-Fold Enhanced Affinity

1ステップ“育種”は30倍超に増強された親和性を持つ診断的抗コルチゾール抗体を作出した

Author (8)： Oyama Hiroyuki (Kobe Pharmaceutical University) ,  Morita Izumi (Kobe Pharmaceutical University) ,  Kiguchi Yuki (Kobe Pharmaceutical University) ,  Morishita Tomomi (Kobe Pharmaceutical University) ,  Fukushima Sakiko (Kobe Pharmaceutical University) ,  Nishimori Yuki (Kobe Pharmaceutical University) ,  Niwa Toshifumi (Department of Medical Technology, School of Health Sciences, Tohoku University) ,  Kobayashi Norihiro (Kobe Pharmaceutical University)
Material： Biological and Pharmaceutical Bulletin  (Biological & Pharmaceutical Bulletin)
Volume： 40  Issue： 12  Page： 2191-2198(J-STAGE)  Publication year： 2017 
JST Material Number： S0989A  ISSN： 0918-6158  CODEN： BPBLEO  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： serum concentration ,...

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Author keywords (12)： antibody ,  single-chain Fv fragment ,  cortisol ,  in vitro evolution ,  affinity maturation ,  enzyme-linked immunosorbent assay ,  antibody ,  single-chain Fv fragment ,  cortisol ,  in vitro evolution ,  affinity maturation ,  enzyme-linked immunosorbent assay

JST classification (1)： Production and application of antigens,antibodies,complements  (ED04030W)

Substance index (2)： Cortisol ,  Prednisolone

Reference (36)：

• 1) The Immunoassay Handbook: Theory and applications of ligand binding, ELISA and related techniques, 4th Edition. (Wild D ed.) Elsevier, the Netherlands (2013).
• 2) Köhler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, 256, 495-497 (1975).
• 3) Therapeutic Monoclonal Antibodies: From bench to clinic. (Zhiqiang A ed.) Wiley, Hoboken, NJ, U.S.A. (2009).
• 4) Kobayashi N, Goto J. Noncompetitive immunoassays for small molecules with high sensitivity and specificity. Adv. Clin. Chem., 36, 139-170 (2001).
• 5) Kobayashi N, Oyama H. Antibody engineering toward high-sensitivity high-throughput immunosensing of small molecules. Analyst, 136, 642-651 (2011).

Terms in the title (6)： 育種 ,  増強 ,  親和性 ,  診断 ,  コルチゾール ,  抗体