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J-GLOBAL ID：201802288127890249   Reference number：18A1892832

Evaluation of internal control genes for quantitative realtime PCR analyses for studying fruit development of dwarf tomato cultivar ‘Micro-Tom’

矮性トマト品種「Micro-Tom」の果実発育を研究するための定量的リアルタイムPCR分析用の内部制御遺伝子の評価

Author (7)： Choi Seung-won (Department of Natural Sciences, International Christian University (ICU)) ,  Hoshikawa Ken (Faculty of Life and Environmental Sciences, University of Tsukuba) ,  Fujita Satoshi (Graduate School of Life and Environmental Sciences, University of Tsukuba) ,  Thi Dung Pham (Graduate School of Life and Environmental Sciences, University of Tsukuba) ,  Mizoguchi Tsuyoshi (Department of Natural Sciences, International Christian University (ICU)) ,  Ezura Hiroshi (Faculty of Life and Environmental Sciences, University of Tsukuba) ,  Ito Emi (Department of Natural Sciences, International Christian University (ICU))
Material： Plant Biotechnology  (Plant Biotechnol)
Volume： 35  Issue： 3  Page： 225-235(J-STAGE)  Publication year： 2018 
JST Material Number： L0316A  ISSN： 1342-4580  CODEN： PLBIF6  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *tomato ,...
Semi thesaurus term： Micro-Tom ,...

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Author keywords (10)： fruit development ,  internal control gene ,  Micro-Tom ,  qRT-PCR ,  reference gene ,  fruit development ,  internal control gene ,  Micro-Tom ,  qRT-PCR ,  reference gene

JST classification (2)： Vegetables  (FC03043X) ,  Gene expression  (EC02040Q)

Reference (31)：

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• Andersen CL, Jensen JL, Ørntoft TF (2004) Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64: 5245-5250
• Argyropoulos D, Psallida C, Spyropoulos CG (2006) Generic normalization method for real-time PCR. Application for the analysis of the mannanase gene expressed in germinating tomato seed. FEBS J 273: 770-777
• Bemer M, Karlova R, Ballester AR, Tikunov YM, Bovy AG, Wolters-Arts M, Rossetto PB, Angenent GC, de Maagd RA (2012) The tomato FRUITFULL homologs TDR4/FUL1 and MBP7/FUL2 regulate ethylene-independent aspects of fruit ripening. Plant Cell 24: 4437-4451
• Brunner AM, Yakovlev IA, Strauss SH (2004) Validating internal controls for quantitative plant gene expression studies. BMC Plant Biol 4: 14

Terms in the title (11)： 矮性 ,  トマト ,  品種 ,  Micro-Tom ,  果実 ,  発育 ,  研究 ,  定量的リアルタイムPCR ,  分析 ,  制御遺伝子 ,  評価