Art

J-GLOBAL ID：201802298162900759   Reference number：18A1037260

Accurate fidelity analysis of the reverse transcriptase by a modified next-generation sequencing

改良次世代シークエンシングによる逆転写酵素の正確な忠実度解析【JST・京大機械翻訳】

Author (10)： Okano Hiroyuki (Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan) ,  Baba Misato (Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan) ,  Hidese Ryota (Department of Bioscience, School of Science and Technology, Kwansei-Gakuin University, 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan) ,  Iida Kei (Medical Research Support Center, Graduate School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan) ,  Li Tongyang (Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan) ,  Kojima Kenji (Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan) ,  Takita Teisuke (Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan) ,  Yanagihara Itaru (Developmental Medicine, Research Institute, Osaka Women’s and Children’s Hospital, 840 Murodocho, Izumi, Osaka, 594-1101, Japan) ,  Fujiwara Shinsuke (Department of Bioscience, School of Science and Technology, Kwansei-Gakuin University, 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan) ,  Yasukawa Kiyoshi (Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan)
Material： Enzyme and Microbial Technology  (EMT)
Volume： 115  Page： 81-85  Publication year： 2018 
JST Material Number： A0989B  ISSN： 0141-0229  Document type： Article
Article type： 原著論文  Country of issue： United States (USA)  Language： ENGLISH (EN)

Abstract/Point： We evaluated fidelity of vario...

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Thesaurus term： DNA polymerase ,  cDNA ,  Retroviridae ,  gene manipulation ,  error rate ,  optimum condition ,  *reverse transcriptase ,  HIV1
Semi thesaurus term： ダイレクトシークエンス法 ,  高濃度 ,  反応条件 ,  ランダム化 ,  *次世代シークエンシング , 【Automatic Indexing@JST】

Author keywords (5)： cDNA synthesis ,  Error rate ,  Fidelity ,  Next-generation sequencing ,  Reverse transcriptase

JST classification (2)： Applications of enzymes  (EB09100B) ,  Enzyme in general  (EB09010D)

Terms in the title (4)： 次世代シークエンシング ,  逆転写酵素 ,  忠実度 ,  解析