Sakaguchi Kenichiro

• Gifu University  Division of Animal Medical Science, Center for One Medicine Innovative Translational Research, Institute for Advanced Study    Associate Professor
• Gifu University  Joint Graduate School of Veterinary Sciences   Associate Professor

• 2025 - 2032 牛卵子の体外発育培養による「種メス牛の育種」の創成
• 2025 - 2028 経腟採卵普及のための牛卵子輸送法開発事業
• 2025 - 2028 Development of in vitro growth culture for bovine oocyte for a future where breeding cows are no longer needed
• 2025 - 2027 卵胞内部に有効成分を浸透させうる新たな牛二次卵胞体外発育培養法の開発
• 2025 - 2027 卵胞内部に有効成分を浸透させうる新たな牛二次卵胞体外発育培養法の開発
2025 - 2026 ゲノム編集による牛胚生産量を爆増させる卵巣巨大化法の開発
2024 - 2025 Development of transportation system for bovine oocytes by controlling meiosis (II)
2020 - 2025 Effects of ovarian reserve on primordial follicle activation and early follicular growth in cattle
2023 - 2024 Development of transportation system for bovine oocytes by controlling meiosis
2021 - 2023 Optimisation of bovine in vitro growth culture based on metabolomics and its app lication to human and conservation medicine
2020 - 2021 Development of in vitro growth (IVG) culture system for bovine oocytes based on metabolomics
2018 - 2020 Development of in vitro follicular growth model based on in vivo follicular development in cattle with different ovarian reserve
2016 - 2019 The attempt to improve the developmental competence of oocytes using in vitro growth model of bovine follicles and the application for the production of wildlife oocytes
2017 - 2018 卵巣予備能の異なる雌牛の体内での卵胞発育動態に基づく体外卵胞発育モデルの開発

• Kohei Kawano, Kenichiro Sakaguchi, Yojiro Yanagawa, Seiji Katagiri. In vivo ovarian temperature promotes the in vitro growth and developmental competence of oocytes derived from bovine early antral follicles. Theriogenology. 2025. 117371-117371
• Kenichiro SAKAGUCHI. Optimization of ovum pick-up-in vitro fertilization and in vitro growth of immature oocytes in ruminants. Journal of Reproduction and Development. 2025. 71. 1. 1-9
• Kohei KAWANO, Kenichiro SAKAGUCHI, Eri FURUKAWA, Nattapong NINPETCH, Yojiro YANAGAWA, Seiji KATAGIRI. Effects of heat stress on gene expression associated with antioxidant capacity of bovine early antral follicles. Journal of Veterinary Medical Science. 2025. 87. 6. 680-684
• Noriyoshi Manabe, Yoichiro Hoshino, Takehiro Himaki, Kenichiro Sakaguchi, Seiji Matsumoto, Tokunori Yamamoto, Tetsuma Murase. Lysate of bovine adipose-derived stem cells accelerates in-vitro development and increases cryotolerance through reduced content of lipid in the in vitro fertilized embryos. Biochemical and Biophysical Research Communications. 2024. 735. 150834-150834
• Kohei KAWANO, Kenichiro SAKAGUCHI, Nattapong NINPETCH, Yojiro YANAGAWA, Seiji KATAGIRI. Physiological high temperatures alter the amino acid metabolism of bovine early antral follicles. Journal of Reproduction and Development. 2024. 70. 3. 184-191

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• 谷田孝志, 池田侑樹, 田嶋彩野, 田嶋彩野, 菅野智裕, 菅野智裕, 坂口謙一郎, 柳川洋二郎, 永野昌志, 永野昌志, et al. 乳用牛の発情後期および発情休止期における耐糖能の違い. 日本獣医学会学術集会講演要旨集. 2023. 166th
• 坂口謙一郎, 永野昌志. 体内・体外における卵子品質向上のための卵胞刺激ホルモン投与と成熟前培養 3. 成熟前培養 (Pre-IVM) による卵子発生能の向上. 繁殖技術. 2021. 41. 4. 81-86
• 坂口謙一郎, 永野昌志. 体内・体外における卵子品質向上のための卵胞刺激ホルモン投与と成熟前培養 2.経腟採卵前の卵胞刺激ホルモン投与法の簡略化. 繁殖技術. 2021. 41. 3. 53-58
• Kenichiro Sakaguchi, Masashi Nagano. Administration of follicle-stimulating hormone and pre-maturation culture to improve oocyte quality in vivo and in vitro 1. Collecting in vivo matured oocytes with higher developmental competence. Technic for Animal Reproduction. 2021. 41. 2. 25-29
• Kenichiro Sakaguchi. Does the number of follicles counted by ultrasonography affect fertility in cattle? -Relationship between the antral follicle count in ovaries and oocyte developmental competence in cattle-. Bull Soc Hokkaido Bov ET. 2020. 39. 1-8

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• 卵子保存又は輸送方法

• Bovine Embryo Transfer for Sustainable Development
  (International Symposium on the Impact of Chemical and Biological Hazards on the Environment, Animals and Human Health and its Countermeasures - Kick off meeting for ZAZINAMBO - 2025)
• Investigation of holding temperatures to establish transport conditions for bovine immature oocytes
  (The 133rd anual meeting of Japanese Society of Animal Science 2025)
• Effects of osteopontin treatment outside the day of estrus on normalization of EGF concentration and fertility
  (The 168th meeting of the Japanese Society of Veterinary Science 2025)
• A Toll-like receptor 4-mediated regulation of EGF production in the bovine uterine cell by palmitic acid
  (The 168th meeting of the Japanese Society of Veterinary Science 2025)
• 全ての肉牛を乳牛が産む未来を切り拓く-牛卵子・卵胞の体外発育培養の最適化-
  (令和6年度東海畜産学会大会シンポジウム「新しいウシの繁殖技術」 2024)

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• 2016 - 2019 Hokkaido University Graduate School of Veterinary Medicine
• 2007 - 2013 Hokkaido University School of Veterinary Medicine

• 博士 (獣医学) (北海道大学)

• 2025/01 - 現在 Hokkaido University Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine Visiting Researcher
• 2024/10 - 現在 Gifu University Joint Graduate School of Veterinary Sciences Associate Professor
• 2024/04 - 現在 Division of Animal Medical Science, Center for One Medicine Innovative Translational Research, Institute for Advanced Study Associate Professor
• 2024/02 - 現在 Gifu University Laboratory of Veterinary Theriogenology, Joint Department of Veterinary Medicine, Faculty of Applied Biological Sciences
• 2023/04 - 2024/01 Hokkaido University Laboratory of Theriogenology, Department of Clinical Sciences, Faculty of Veterinary Medicine Postdoctoral Fellow
2019/04 - 2024/01 Hokkaido University Laboratory of Theriogenology, Department of Clinical Sciences, Faculty of Veterinary Medicine Visiting Researcher
2021/04 - 2023/03 Japan Society for the Promotion of Science Overseas Research Fellow
2019/04 - 2023/03 University of Edinburgh Institute of Cell Biology, School of Biological Sciences Sponsored Researcher
2020/04 - 2021/03 上原記念生命科学財団 ポストドクトラルフェロー
2020/05 - 2020/11 Hokkaido University Department of Clinical Sciences, Division of Veterinary Medicine, Faculty of Veterinary Medicine Research Fellow
2019/04 - 2020/03 Japan Society for the Promotion of Science Reserch Fellow (PD-Fellowship)
2018/04 - 2019/03 Japan Society for the Promotion of Science Reserch Fellow (DC2-fellowship)
2013/04 - 2016/03 Zen-noh ET Center Reserch and Development Group Veterinarian

• 2025/06 - 現在 Journal of Visualized Experiments(JoVE) Guest Editor of Methods Collections "Cutting-Edge Strategies for Utilizing Immature Oocytes"
• 2025/04 - 現在 岐阜県獣医師会 組織財政委員会委員
• 2025/01 - 現在 日本繁殖生物学会 プログラム委員会委員
• 2024/09 - 2026/09 日本獣医学会 獣医繁殖学分科会 国際交流委員会委員
• 2024/11 - 2025/02 Graduate School of Veterinary Sciences, Kitasato University Thesis Review Committee Member (Associate Examiner)
2021/06 - 2024/03 Animals Guest Editor of Special Issue "In Vitro Growth of Mammalian Follicles and Oocytes"
2017/01 - 2017/09 Organizer of The Student Session at The 5th Sapporo Summer Seminar for One Health

• 2025/11 - 公益財団法人 日本農学会 2025年度 (第24回) 日本農学進歩賞 牛における卵胞機能障害の病態解明と新規体外発育培養系への応用
• 2025/11 - 公益社団法人 日本獣医学会 第168回日本獣医学会 学術集会優秀発表賞(獣医繁殖学分科会) A Toll-like receptor 4-mediated regulation of EGF production in the bovine uterine cell by palmitic acid
• 2024/09 - 公益社団法人 日本繁殖生物学会 日本繁殖生物学会賞・奨励賞 反芻動物における経腟採卵-体外受精および未成熟卵子の体外発育培養の最適化に関する研究
• 2024/09 - The Japanese Society of Veterinary Science Veterinary Science Young Investigator Award Pathophysiological study on follicular dysfunction by in vivo and in vitro growing bovine follicle
• 2023/09 - 日本獣医学会 学術集会優秀発表賞(獣医繁殖学分科会)
2022/06 - Wiley ANIMAL SCIENCE JOURNAL Top Downloaded Article Effects of follicle-stimulating hormone followed by gonadotropin-releasing hormone on embryo production by ovum pick-up and in vitro fertilization in the river buffalo (Bubalus bubalis)
2021/09 - The Subcommittee of Theriogenology of the Japanese Society of Veterinary Science Best presentation award Effects of the presence of a dominant follicle and a corpus luteum in bovine ovaries on nuclear maturation of oocytes and steroidogenesis of granulosa cells cultured derived from early antral follicles
2021/01 - International Embryo Technology Society Peter W. Farin Trainee Travel Scholarship Award 2021 The effects of heat exposure on the growth and developmental competence of oocytes derived from early antral follicles in dairy cows
2019/03 - Graduate School of Veterinary Medicine, Hokkaido University The representative of the graduates of the Graduate School of Veterinary Medicine
2018/09 - The Subcommittee of Theriogenology of the Japanese Society of Veterinary Science Best presentation award Relationship between antral follicle count, granulosa cell proliferation and oocyte developmental competence of oocyte-granulosa cell complexes derived from early antral follicles in cattle
2017/09 - The Subcommittee of Theriogenology of the Japanese Society of Veterinary Science Best presentation award Relationship between antral follicle count and steroidogenesis of oocyte-granulosa cell complexes derived from early antral follicles in cattle
2015/11 - The Hokkaido Artificial Insemination Technician Association Presentation award For the development of the top Wagyu herd-Production of embryos by OPU-IVF using cattle with high breeding value-

Japanese Society of Animal Science ,  Gifu Veterinary Medical Association ,  Japan Veterinary Medical Association ,  Artificial Insemination Association of Gifu ,  Artificial Insemination Association of Japan (AIAJ) ,  Society for Reproduction and Fertility ,  The International Embryo Technology Society ,  The Japanese Society of Veterinary Science ,  SOCIETY FOR REPRODUCTION AND DEVELOPMENT