Art

J-GLOBAL ID：201902221522003287   Reference number：19A1259878

Purification of insoluble GST-fused and GST-cleaved Cav1.2 channel fragment by denaturation and renaturation

変性及び再生による不溶性GST融合及びGST切断Cav1.2チャンネル断片の精製【JST・京大機械翻訳】

Author (4)： Gao Qinghua (Department of Physiology, Graduate School of Medical & Dental Sciences, Kagoshima University, Kagoshima, 890-8544, Japan) ,  Minobe Etsuko (Department of Physiology, Graduate School of Medical & Dental Sciences, Kagoshima University, Kagoshima, 890-8544, Japan) ,  Kameyama Masaki (Department of Physiology, Graduate School of Medical & Dental Sciences, Kagoshima University, Kagoshima, 890-8544, Japan) ,  Xu Jianjun (Department of Physiology, Graduate School of Medical & Dental Sciences, Kagoshima University, Kagoshima, 890-8544, Japan)
Material： Protein Expression and Purification  (Protein Expression & Purification)
Volume： 160  Page： 7-10  Publication year： 2019 
JST Material Number： W0282A  ISSN： 1046-5928  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Abstract/Point： Both recombinant glutathione-S...

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Thesaurus term： peptide bond hydrolase ,  granular material ,  solubility(property) ,  fusion protein ,  heart ,  *channel ,  biological activity ,  interaction ,  guinea pig ,  glutathione transferase ,  solubilization ,  Escherichia coli
Semi thesaurus term： C末端 ,  カルジオトロフィン1 ,  *Cav1.2 , 【Automatic Indexing@JST】

Author keywords (5)： Calcium channel ,  Glutathione-S-Transferase fusion protein ,  Denaturation ,  Renaturation ,  Pull-down assay

JST classification (2)： Gene expression  (EC02040Q) ,  Gene manipulation  (EC02050B)

Terms in the title (7)： 変性 ,  不溶性 ,  GST ,  融合 ,  2チャンネル ,  断片 ,  精製