Art

J-GLOBAL ID：201902230790982016   Reference number：19A1861310

Construction of an All-in-one Double-conditional shRNA Expression Vector

オールインワン二重支配型shRNA発現ベクターの構築

Author (11)： KUDO Yoshiko (Department of Pharmacology, Showa University School of Medicine) ,  KUDO Yoshiko (International University of Health and Welfare, School of Health Science, Department of Medical Science Technology) ,  OYAMADA Hideto (Department of Pharmacology, Showa University School of Medicine) ,  MATSUOKA Tomoyuki (Department of Pharmacology, Showa University School of Medicine) ,  INAGAKI Ayami (Department of Pharmacology, Showa University School of Medicine) ,  TSUCHIYA Hiromichi (Department of Pharmacology, Showa University School of Medicine) ,  AO Yoko (Department of Pharmacology, Showa University School of Medicine) ,  KIMURA Atsushi Michael (Department of Pharmacology, Showa University School of Medicine) ,  TSUJI Mayumi (Department of Pharmacology, Showa University School of Medicine) ,  OGUCHI Katsuji (Department of Pharmacology, Showa University School of Medicine) ,  KIUCHI Yuji (Department of Pharmacology, Showa University School of Medicine)
Material： Showa University Journal of Medical Sciences  (Showa Univ J Med Sci)
Volume： 31  Issue： 1  Page： 41-50(J-STAGE)  Publication year： 2019 
JST Material Number： L1154A  ISSN： 0915-6380  CODEN： SUMSEG  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： transgenic mouse ,...
Semi thesaurus term： genetic regulation ,...

Register(free) or Log In to access     To see more with JDream III (charged).   {{ this.onShowAbsJLink("http://jdream3.com/lp/jglobal/index.html?docNo=19A1861310&from=J-GLOBAL&jstjournalNo=L1154A") }}

Author keywords (5)： RNA interference ,  short hairpin RNA ,  double-conditional vector ,  tetracycline-inducible system ,  Cre-loxP system

JST classification (1)： Research methods in genetics  (EC01020N)

Reference (14)：

• 1) Elbashir SM, Harborth J, Lendeckel W, et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 2001;411:494-498.
• 2) Brummelkamp TR, Bernards R, Agami R. A system for stable expression of short interfering RNAs in mammalian cells. Science. 2002;296:550-553.
• 3) Seibler J, Kuter-Luks B, Kern H, et al. Single copy shRNA configuration for ubiquitous gene knockdown in mice. Nucleic Acids Res. 2005;33:e67. (accessed 2018 Dec 20) Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1079974/pdf/gni065.pdf
• 4) Ko JK, Choi KH, Zhao X, et al. A versatile single-plasmid system for tissue-specific and inducible control of gene expression in transgenic mice. FASEB J. 2011;25:2638-2649.
• 5) Kuhn R, Streif S, Wurst W. RNA interference in mice. Handb Exp Pharmacol. 2007;178:149-176.

Terms in the title (4)： 二重支配 ,  shRNA ,  発現ベクター ,  構築