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J-GLOBAL ID：201902252753485145   Reference number：19A0217469

Design and prototyping of a Bombyx mori nucleopolyhedrovirus-based genome assembly from PCR-amplified DNA fragments

PCR増幅DNA断片からのカイコ核多角体ウイルスに基づくゲノムアセンブリの設計とプロトタイピング

Author (5)： Harada Naoto (Laboratory of Applied Molecular Entomology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University) ,  Ishibashi Daiki (Laboratory of Applied Molecular Entomology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University) ,  Asano Shin-ichiro (Laboratory of Applied Molecular Entomology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University) ,  Sato Masanao (Laboratory of Applied Molecular Entomology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University) ,  Bando Hisanori (Laboratory of Applied Molecular Entomology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University)
Material： Journal of Insect Biotechnology and Sericology  (J Insect Biotechnol Sericology)
Volume： 87  Issue： 3  Page： 3_097-3_108(J-STAGE)  Publication year： 2018 
JST Material Number： L4393A  ISSN： 1346-8073  CODEN： NISZAQ  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *nuclear polyhedrosis virus ,...
Semi thesaurus term： *カイコ核多角体ウイルス ,...

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Author keywords (3)： Bombyx mori nucleopolyhedrovirus ,  DNA assembly ,  Synthetic biology

JST classification (2)： Research methods in microbiology and virology in general  (EG02010V) ,  Gene manipulation  (EC02050B)

Reference (21)：

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• Gibson, D.G., Benders, G.A., Axelrod, K.C., Zaveri, J., Algire, M.A., Moodie, M., Montague, M.G., Venter, J.C., Smith, H.O. and Hutchison, C.A. 3rd (2008b) One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome. Proc. Natl. Acad. Sci. USA., 105, 20404-20409.
• Gibson, D.G., Young, L., Chuang, R.Y., Venter, J.C., Hutchison, C.A. 3rd and Smith, H.O. (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat. Methods, 6, 343-345.

Terms in the title (7)： PCR増幅 ,  DNA断片 ,  カイコ ,  核多角体ウイルス ,  ゲノムアセンブリ ,  設計 ,  プロトタイピング