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J-GLOBAL ID：201902255419525043   Reference number：19A2356410

Purification and enzymatic characterization of trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from Rhodococcus opacus and enzymatic formation of α, β-unsaturated ketones

Rhodococcus opacus由来のトランス-o-ヒドロキシベンジリデンピルビン酸ヒドラターゼ-アルドラーゼの精製と酵素的特性化及びα,β-不飽和ケトン類の酵素的生成

Author (2)： SUZUKI Toshihiro (Tokyo Univ. Agriculture, Tokyo, JPN) ,  TAKIZAWA Noboru (Okayama Univ. Sci., Okayama, JPN)
Material： Bioscience, Biotechnology, and Biochemistry  (Bioscience Biotechnology & Biochemistry)
Volume： 83  Issue： 10  Page： 1884-1888  Publication year： Oct. 2019 
JST Material Number： G0021A  ISSN： 0916-8451  CODEN： BBBIEJ  Document type： Article
Article type： 短報  Country of issue： United Kingdom (GBR)  Language： ENGLISH (EN)

Thesaurus term： *aldolase ,...
Semi thesaurus term： *Rhodococcus ,...

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JST classification (3)： Enzyme in general  (EB09010D) ,  Aromatic monocyclic ketones  (CF05170I) ,  Microorganism biochemistry  (EG03020N)

Substance index (3)： Salicylaldehyde ,  Pyruvic Acid ,  (E)-2-Hydroxybenzylidenepyruvic acid

Reference (10)：

• Eaton RW. Organization and evolution of naphthalene catabolic pathways: sequence of the DNA encoding 2-hydroxychromene-2-carboxylate isomerase and trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from the NAH7 plasmid. J Bacteriol. 1994;176(24):7757-7762.
• Ferrara S, Mapelli E, Sello G, et al. Characterization of the aldol condensation activity of the trans-o-hydroxybenzylidenepyruvate hydratase-aldolase (tHBP-HA) cloned from Pseudomonas fluorescens N3. Biochim Biophys Acta. 2011;1814(5):622-629.
• Sello G, Di Gennaro P. Aldol reaction of the trans-o-hydroxybenzylidenepyruvate hydratase-aldolase (tHBP-HA) from Pseudomonas fluorescens N3. Appl Biochem Biotechnol. 2013;170(7):1702-1712.
• Eaton RW, Chapman PJ. Bacterial metabolism of naphthalene: construction and use of recombinant bacteria to study ring cleavage of 1,2-dihydroxynaphthalene and subsequent reactions. J Bacteriol. 1992;174(23):7542-7554.
• Eaton RW. trans-o-hydroxybenzylidenepyruvate hydratase-aldolase as a biocatalyst. Appl Environ Microbiol. 2000;66(6):2668-2672.

Terms in the title (9)： Rhodococcus ,  トランス ,  酸 ,  ヒドラターゼ ,  アルドラーゼ ,  精製 ,  酵素 ,  特性化 ,  不飽和ケトン類