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J-GLOBAL ID：201902256234425009   Reference number：19A1285749

Improved 19F{1H} Saturation Transfer Difference Experiments for Sensitive Detection to Fluorinated Compound Bound to Proteins

蛋白質に結合したフッ素化化合物への高感度検出のための改良19F{1H}飽和移動差実験【JST・京大機械翻訳】

Author (2)： Furihata Kazuo (Division of Agriculture and Agricultural Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan) ,  Tashiro Mitsuru (Department of Chemistry, College of Science and Technology, Meisei University, Hino, Tokyo 191-8506, Japan)
Material： Magnetochemistry (Web)
Volume： 5  Issue： 1  Page： 1  Publication year： 2018 
JST Material Number： U7235A  ISSN： 2312-7481  Document type： Article
Article type： 短報  Country of issue： Switzerland (CHE)  Language： ENGLISH (EN)

Abstract/Point： The 19F{1H} saturation transfe...

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Thesaurus term： *high sensitivity ,  screening(inspection) ,  detection method ,  *fluorination ,  pulse ,  *protein ,  NMR ,  complex(substance)
Semi thesaurus term： 実用性 ,  ヒト血清アルブミン , 【Automatic Indexing@JST】

Author keywords (5)： NMR-based screening ,  fluorinated compound ,  diflunisal ,  ¹⁹F NMR ,  shaped pulses

JST classification (5)： Enzyme in general  (EB09010D) ,  Proteins and peptides in general  (EB03010N) ,  NMR of organic compounds  (BM07044Q) ,  Analytical instruments  (CC02020S) ,  Biophysical research methods  (EA03020V)

Reference (16)：

• Chen, A.; Shapiro, M.J. NOE Pumping: A novel NMR technique for identification of compounds with binding affinity to macromolecules. J. Am. Chem. Soc. 1998, 120, 10258-10259.
• Maye, M.; Meyer, B. Group epitope mapping by saturation transfer difference NMR to identify segments of a ligand in direct contact with a protein receptor. J. Am. Chem. Soc. 2001, 123, 6108-6117.
• Dalvit, C.; Pevarello, P.; Tatò, M.; Veronesi, M.; Vulpetti, A.; Sundström, M. Identification of compounds with binding affinity to proteins via magnetization transfer from bulk water. J. Biomol. NMR 2000, 18, 65-68.
• Dalvit, C.; Fogliatto, G.P.; Stewart, A.; Veronesi, M.; Stockman, B.J. WaterLOGSY as a method for primary NMR screening: Practical aspects and range of applicability. J. Biomol. NMR 2001, 21, 349-359.
• Chen, A.; Shapiro, M.J. NOE Pumping. 2. A high-throughput method to determine compounds with binding affinity to macromolecules by NMR. J. Am. Chem. Soc. 2000, 122, 414-415.

Terms in the title (4)： 蛋白質 ,  フッ素化 ,  高感度 ,  実験