Art

J-GLOBAL ID：201902269219305127   Reference number：19A0148827

Generation of PDX-1 mutant porcine blastocysts by introducing CRISPR/Cas9-system into porcine zygotes via electroporation

エレクトロポレーションを介したブタ接合子へのCRISPR/Cas9-システム導入によるPDX-1変異ブタ胚盤胞の作製

Author (9)： Tanihara Fuminori (Laboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University, Ishii-cho, Tokushima, Japan) ,  Hirata Maki (Laboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University, Ishii-cho, Tokushima, Japan) ,  Nguyen Nhien T. (Laboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University, Ishii-cho, Tokushima, Japan) ,  Le Quynh A. (Laboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University, Ishii-cho, Tokushima, Japan) ,  Hirano Takayuki (Laboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University, Ishii-cho, Tokushima, Japan) ,  Takemoto Tatsuya (Division of Embryology, Institute of Advanced Medical Sciences, Tokushima University, Kuramoto-cho, Tokushima, Japan) ,  Nakai Michiko (Division of Animal Sciences, Animal Biotechnology Unit, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan) ,  Fuchimoto Dai-ichiro (Division of Animal Sciences, Animal Biotechnology Unit, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan) ,  Otoi Takeshige (Laboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University, Ishii-cho, Tokushima, Japan)
Material： Animal Science Journal  (Anim Sci J)
Volume： 90  Issue： 1  Page： 55-61  Publication year： Jan. 2019 
JST Material Number： W1430A  ISSN： 1344-3941  Document type： Article
Article type： 原著論文  Country of issue： United States (USA)  Language： ENGLISH (EN)

Thesaurus term： *electroporation ,...
Semi thesaurus term： CRISPR-Cas Systems ,...

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Author keywords (5)： CRISPR/Cas9 ,  genome editing ,  in vitro fertilization ,  pig zygote ,  zygote electroporation

JST classification (3)： Swine(=pigs)  (FD02040U) ,  Development(=generation)and differentiation  (EJ16020W) ,  Gene expression  (EC02040Q)

Terms in the title (10)： エレクトロポレーション ,  ブタ ,  接合子 ,  CRISPR ,  Cas9 ,  システム導入 ,  PDX-1 ,  変異 ,  胚盤胞 ,  作製