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J-GLOBAL ID：202002215264450029   Reference number：20A2795562

3D Culture Models with CRISPR Screens Reveal Hyperactive NRF2 as a Prerequisite for Spheroid Formation via Regulation of Proliferation and Ferroptosis

CRISPRスクリーンによる3D培養モデルは増殖およびフェロトーシスの調節を介してスフェロイド形成の必要条件として超活性NRF2を明らかにする【JST・京大機械翻訳】

Author (17)： Takahashi Nobuaki (Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA) ,  Takahashi Nobuaki (Ludwig Cancer Center, Boston, MA 02115, USA) ,  Cho Patricia (Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA) ,  Cho Patricia (Ludwig Cancer Center, Boston, MA 02115, USA) ,  Selfors Laura M. (Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA) ,  Selfors Laura M. (Ludwig Cancer Center, Boston, MA 02115, USA) ,  Kuiken Hendrik J. (Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA) ,  Kuiken Hendrik J. (Ludwig Cancer Center, Boston, MA 02115, USA) ,  Kaul Roma (Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA) ,  Kaul Roma (Ludwig Cancer Center, Boston, MA 02115, USA) ,  Fujiwara Takuro (Department of Synthetic Chemistry and Biological Chemistry, Kyoto University, Kyoto 615-8510, Japan) ,  Harris Isaac S. (Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA) ,  Harris Isaac S. (Ludwig Cancer Center, Boston, MA 02115, USA) ,  Zhang Tian (Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA) ,  Gygi Steven P. (Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA) ,  Brugge Joan S. (Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA) ,  Brugge Joan S. (Ludwig Cancer Center, Boston, MA 02115, USA)
Material： Molecular Cell  (Mol Cell)
Volume： 80  Issue： 5  Page： 828-844.e6  Publication year： 2020 
JST Material Number： W1167A  ISSN： 1097-2765  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Abstract/Point： Cancer-associated mutations th...

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Thesaurus term： *screen ,  tumor ,  tumor cell ,  Mutation ,  peroxidase ,  *three dimension ,  targeting ,  lung tumor ,  Down-Regulation ,  transcription factor ,  pathogenesis ,  proteomics ,  antioxidants ,  *Cellular Spheroids ,  peroxide oxidoreductase
Semi thesaurus term： *スフェロイド , 【Automatic Indexing@JST】

Author keywords (1)： oxidative stress cancer cell death ferroptosis 3D culture NRF2 CRISPR screening selenoprotein GPX4

JST classification (3)： Chemistry,biochemistry and pathology of tumors(=neoplasms)  (GE02030N) ,  Cell physiology in general  (EF02010S) ,  Basic oncology in general  (GE02010R)

Terms in the title (11)： CRISPR ,  スクリーン ,  3D ,  培養 ,  モデル ,  増殖 ,  フェロトーシス ,  調節 ,  スフェロイド ,  必要条件 ,  NRF2