Art

J-GLOBAL ID：202002218065220580   Reference number：20A1762150

Generation of novel Il2rg-knockout mice with clustered regularly interspaced short palindromic repeats (CRISPR) and Cas9

Clustered regularly interspaced short palindromic repeats(CRISPR)とCas9による新たなIl2rg-ノックアウトマウスの作出

Author (15)： Byambaa Suvd (Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan) ,  Uosaki Hideki (Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan) ,  Uosaki Hideki (Center for Development of Advanced Medical Technology, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan) ,  Hara Hiromasa (Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan) ,  Hara Hiromasa (Center for Development of Advanced Medical Technology, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan) ,  Nagao Yasumitsu (Center for Development of Advanced Medical Technology, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan) ,  Nagao Yasumitsu (Center for Experimental Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan) ,  Abe Tomoyuki (Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan) ,  Abe Tomoyuki (Center for Development of Advanced Medical Technology, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan) ,  Shibata Hiroaki (Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan) ,  Shibata Hiroaki (Center for Development of Advanced Medical Technology, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan) ,  Nureki Osamu (Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan) ,  Ohmori Tsukasa (Department of Biochemistry, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan) ,  Hanazono Yutaka (Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan) ,  Hanazono Yutaka (Center for Development of Advanced Medical Technology, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan)
Material： Experimental Animals (Web)
Volume： 69  Issue： 2  Page： 189-198(J-STAGE)  Publication year： 2020 
JST Material Number： U2149A  ISSN： 1881-7122  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： mouse(animal) ,...
Semi thesaurus term： *CRISPR-Cas9 system ,...

Register(free) or Log In to access     To see more with JDream III (charged).   {{ this.onShowAbsJLink("http://jdream3.com/lp/jglobal/index.html?docNo=20A1762150&from=J-GLOBAL&jstjournalNo=U2149A") }}

Author keywords (5)： animal model ,  clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 ,  genome-editing ,  II2rg ,  X-linked severe combined immunodeficiency (X-SCID)

JST classification (4)： Congenital diseases,deformities in general.  (GD03010V) ,  Immunologic diseases,allergic diseases in general.  (GD04010C) ,  Research methods in genetics  (EC01020N) ,  Laboratry organisms  (EA03070Y)

Reference (36)：

• 1. Bae S., Park J. and Kim J.S. 2014. Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. Bioinformatics 30: 1473-1475.
• 2. Birling M.C., Herault Y. and Pavlovic G. 2017. Modeling human disease in rodents by CRISPR/Cas9 genome editing. Mamm. Genome 28: 291-301.
• 3. Cao X., Shores E.W., Hu-Li J., Anver M.R., Kelsall B.L., Russell S.M., Drago J., Noguchi M., Grinberg A., Bloom E.T., Pau L.W.E., Katz S.I., Love P.E. and Leonard W.J. 1995. Defective lymphoid development in mice lacking expression of the common cytokine receptor gamma chain. Immunity 2: 223-238.
• 4. Cavazzana-Calvo M., Hacein-Bey S., de Saint Basile G., Gross F., Yvon E., Nusbaum P., Selz F., Hue C., Certain S., Casanova J.L., Bousso P., Deist F.L. and Fischer A. 2000. Gene therapy of human severe combined immunodeficiency (SCID)-X1 disease. Science 288: 669-672.
• 5. Chang C.W., Lai Y.S., Westin E., Khodadadi-Jamayran A., Pawlik K.M., Lamb L.S. Jr., Goldman F.D. and Townes T.M. 2015. Modeling human severe combined immunodeficiency and correction by CRISPR/Cas9-enhanced gene targeting. Cell Rep. 12: 1668-1677.

Terms in the title (3)： CRISPR ,  Cas9 ,  ノックアウトマウス