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J-GLOBAL ID：202002219225500008   Reference number：20A1395517

Efficient transient gene expression system using buckwheat hypocotyl protoplasts for large-scale experiments

大規模実験のためのソバ胚軸プロトプラストを用いた効率的な一過性遺伝子発現系

Author (4)： Sakamoto Shingo (Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)) ,  Matsui Katsuhiro (Institute of Crop Science, National Agriculture and Food Research Organization (NARO)) ,  Oshima Yoshimi (Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)) ,  Mitsuda Nobutaka (Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST))
Material： Breeding Science  (Breed Sci)
Volume： 70  Issue： 1  Page： 128-134(J-STAGE)  Publication year： 2020 
JST Material Number： Y0311B  ISSN： 1344-7610  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *Fagopyrum esculentum ,...
Semi thesaurus term： *Transient expression

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Author keywords (5)： buckwheat ,  effector-reporter analysis ,  hypocotyl protoplast ,  transient gene expression ,  96-well plate

JST classification (4)： Gene manipulation  (EC02050B) ,  Research methods in botany  (EH01020C) ,  Plant breeding in general  (FC02010K) ,  Field crops in general  (FC03031E)

Reference (36)：

• Adachi, T., A. Yamaguchi, Y. Miike and F. Hoffmann (1989) Plant regeneration from protoplasts of common buckwheat (Fagopyrum esculentum). Plant Cell Rep. 8: 247-250.
• Agius, F., I. Amaya, M.A. Botella and V. Valpuesta (2005) Functional analysis of homologous and heterologous promoters in strawberry fruits using transient expression. J. Exp. Bot. 56: 37-46.
• Cao, J.M., D.M. Yao, F. Lin and M.Y. Jiang (2014) PEG-mediated transient gene expression and silencing system in maize mesophyll protoplasts: a valuable tool for signal transduction study in maize. Acta Physiol. Plant. 36: 1271-1281.
• Fujikawa, Y. and N. Kato (2007) Split luciferase complementation assay to study protein-protein interactions in Arabidopsis protoplasts. Plant J. 52: 185-195.
• Fujiwara, S., S. Sakamoto, K. Kigoshi, K. Suzuki and M. Ohme-Takagi (2014) VP16 fusion induces the multiple-knockout phenotype of redundant transcriptional repressors partly by Med25-independent mechanisms in Arabidopsis. FEBS Lett. 588: 3665-3672.

Terms in the title (6)： 大規模 ,  実験 ,  ソバ ,  胚軸 ,  プロトプラスト ,  遺伝子発現