Art

J-GLOBAL ID：202002221186533287   Reference number：20A2010807

Expression and characterization of l-arabinose isomerase from Geobacillus stearothermophilus for improved activity under acidic condition

酸性条件下で改良された活性のためのGeobacillus stearothermophilus由来L-アラビノースイソメラーゼの発現と特性化【JST・京大機械翻訳】

Author (8)： Laksmi Fina Amreta (The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24, Korimoto, Kagoshima, 890-0065, Japan) ,  Arai Shigeki (Institute for Quantum Life Science, National Institute for Quantum and Radiological Science and Technology, 2-4 Shirakata, Tokai, Ibaraki, 319-1106, Japan) ,  Arakawa Tsutomu (Alliance Protein Laboratories, a Division of KBI Biopharma, 6042 Cornerstone Court West, Suite A, San Diego, CA, 92121, USA) ,  Tsurumaru Hirohito (The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24, Korimoto, Kagoshima, 890-0065, Japan) ,  Nakamura Yoshitaka (The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24, Korimoto, Kagoshima, 890-0065, Japan) ,  Saksono Budi (Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km. 46, Cibinong, 16911, Indonesia) ,  Tokunaga Masao (The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24, Korimoto, Kagoshima, 890-0065, Japan) ,  Ishibashi Matsujiro (The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24, Korimoto, Kagoshima, 890-0065, Japan)
Material： Protein Expression and Purification  (Protein Expression & Purification)
Volume： 175  Page： Null  Publication year： 2020 
JST Material Number： W0282A  ISSN： 1046-5928  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Abstract/Point： A low-calorie sugar-substituti...

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Thesaurus term： high temperature ,  Maillard Reaction ,  sweetener ,  substrate (biochemistry) ,  Substrate Specificity ,  disulfide bond ,  Proteins ,  binding site ,  secondary structure ,  *acidity (property) ,  wild type ,  *Geobacillus stearothermophilus ,  *L-arabinose isomerase ,  optimal pH ,  *microorganism ,  *Bacillus ,  *Spore-Forming Bacteria
Semi thesaurus term： 残基 , 【Automatic Indexing@JST】

Author keywords (5)： l-arabinose isomerase ,  d-tagatose ,  Acidic adaptation ,  Error-prone PCR ,  pH profile

JST classification (2)： Enzyme in general  (EB09010D) ,  Gene manipulation  (EC02050B)

Terms in the title (6)： 酸性条件 ,  活性 ,  Geobacillus ,  L-アラビノースイソメラーゼ ,  発現 ,  特性化