Art

J-GLOBAL ID：202002235176782414   Reference number：20A1195586

Establishment of efficient Knockin method which gives only desired mutation by genome editing.

ゲノム編集で目的の変異のみを組み込む効率的なノックイン法の確立

Author (1)： UEDA ATSUSHI (金沢大 医薬保健研究域 医学系 再生分子医学)
Material： 東京生化学研究会助成研究報告集
Volume： 33  Page： 129-134  Publication year： Oct. 31, 2019 
JST Material Number： L7935A  ISSN： 1345-4927  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： JAPANESE (JA)

Thesaurus term： *gene manipulation ,...
Semi thesaurus term： Clustered Regularly Interspaced Short Palindromic Repeats ,...

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JST classification (2)： Gene manipulation  (EC02050B) ,  Nucleic acids in general  (EB04010U)

Substance index (1)： Puromycin

Reference (5)：

• Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F: Genome engineering using the CRISPR-Cas9 system. Nat Protoc, 8, 2281-2308 (2013).
• Matsuda T, Nakamura T, Nakao K, Arai T, Katsuki M, Heike T, Yokota T: STAT3 activation is sufficient to maintain an undifferentiated state of mouse embryonic stem cells. EMBO J, 18,4261-4269 (1999).
• Ho L, Miller EL, Ronan JL, Ho WQ, Jothi R, Crabtree GR: esBAF facilitates pluripotency by conditioning the genome for LIF/STAT3 signalling and by regulating polycomb function. Nat Cell Biol, 13, 903-913 (2011).
• Yagi T, Nada S, Watanabe N, Tamemoto H, Kohmura N, Ikawa Y, Aizawa S: A novel negative selection for homologous recombinants using diphtheria toxin A fragment gene. Anal Biochem, 214, 77-86 (1993).
• Yao X, Zhang M, Wang X, Ying W, Hu X, Dai P, Meng F, et al. : Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells. Dev Cell, 45, 526-536 (2018).

Terms in the title (3)： ゲノム編集 ,  変異 ,  ノック