Art

J-GLOBAL ID：202002277481595487   Reference number：20A1551711

A simple and practical workflow for genotyping of CRISPR-Cas9-based knockout phenotypes using multiplexed amplicon sequencing

マルチプレックスアンプリコンシーケンシングを用いたCRISPR-Cas9ベースノックアウト表現型の遺伝子タイピングのための簡単で実用的なワークフロー

Author (9)： Iida Midori (Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Iizuka, Japan) ,  Suzuki Miyuki (Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Japan) ,  Sakane Yuto (Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Japan) ,  Nishide Hiroyo (Laboratory of Genome Informatics (Data Integration and Analysis Facility), National Institute for Basic Biology, Okazaki, Japan) ,  Uchiyama Ikuo (Laboratory of Genome Informatics (Data Integration and Analysis Facility), National Institute for Basic Biology, Okazaki, Japan) ,  Yamamoto Takashi (Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Japan) ,  Suzuki Ken-ichi T. (Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Japan) ,  Suzuki Ken-ichi T. (Center for the Development of New Model Organisms, National Institute for Basic Biology, Okazaki, Japan) ,  Fujii Satoshi (Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Iizuka, Japan)
Material： Genes to Cells  (Genes Cells)
Volume： 25  Issue： 7  Page： 498-509  Publication year： Jul. 2020 
JST Material Number： W0935A  ISSN： 1356-9597  CODEN： GECEFL  Document type： Article
Article type： 原著論文  Country of issue： Australia (AUS)  Language： ENGLISH (EN)

Thesaurus term： *gene manipulation ,...
Semi thesaurus term： *CRISPR-Cas9 method ,...

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Author keywords (7)： amplicon sequencing ,  crispant ,  CRISPR-Cas9 ,  genotyping ,  knockout ,  somatic mutation ,  web tool

JST classification (3)： Research methods in genetics  (EC01020N) ,  Gene manipulation  (EC02050B) ,  Molecular and genetic information processing  (JE15040B)

Terms in the title (7)： マルチプレックス ,  アンプリコンシーケンシング ,  CRISPR-Cas9 ,  ノックアウト ,  表現型 ,  遺伝子タイピング ,  ワークフロー