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J-GLOBAL ID：202002288996248072   Reference number：20A0521949

Fluorescence Signal Amplification by Using β-Galactosidase for Flow Cytometry; Advantages of an Endogenous Activity-Free Enzyme

フローサイトメトリーのためのβ-ガラクトシダーゼ使用による蛍光シグナル増幅 内因性活性フリー酵素の利点【JST・京大機械翻訳】

Author (20)： Nobori Takanobu (Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Japan) ,  Kawamura Masumi (Graduate School of Systems Life Sciences, Kyushu University, Japan) ,  Yoshida Ryosuke (Graduate School of Systems Life Sciences, Kyushu University, Japan) ,  Joichi Taisei (Graduate School of Systems Life Sciences, Kyushu University, Japan) ,  Kamino Kenta (Graduate School of Systems Life Sciences, Kyushu University, Japan) ,  Kishimura Akihiro (Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Japan) ,  Kishimura Akihiro (Graduate School of Systems Life Sciences, Kyushu University, Japan) ,  Kishimura Akihiro (Center for Future Chemistry, Kyushu University, Japan) ,  Kishimura Akihiro (Institute of Systems, Information Technologies and Nanotechnologies, Japan) ,  Baba Eishi (Department of Cmprehensive Clinical Oncology, Faculty of Medical Sciences, Kyushu University, Japan) ,  Mori Takeshi (Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Japan) ,  Mori Takeshi (Graduate School of Systems Life Sciences, Kyushu University, Japan) ,  Mori Takeshi (Center for Future Chemistry, Kyushu University, Japan) ,  Katayama Yoshiki (Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Japan) ,  Katayama Yoshiki (Graduate School of Systems Life Sciences, Kyushu University, Japan) ,  Katayama Yoshiki (Center for Future Chemistry, Kyushu University, Japan) ,  Katayama Yoshiki (Institute of Systems, Information Technologies and Nanotechnologies, Japan) ,  Katayama Yoshiki (International Research Center for Molecular Systems, Kyushu University, Japan) ,  Katayama Yoshiki (Center for Advanced Medical Innovation, Kyushu University, Japan) ,  Katayama Yoshiki (Department of Biomedical Engineering, Chung Yuan Christian University, Taiwan)
Material： Analytical Chemistry  (Anal Chem)
Volume： 92  Issue： 4  Page： 3069-3076  Publication year： 2020 
JST Material Number： A0395A  ISSN： 0003-2700  CODEN： ANCHAM  Document type： Article
Article type： 原著論文  Country of issue： United States (USA)  Language： ENGLISH (EN)

Abstract/Point： We previously proposed using a...

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Thesaurus term： *enzyme ,  catalyst ,  dyeing ,  substrate (biochemistry) ,  Proteins ,  Osmosis ,  antigen ,  Cell Membrane ,  Alkaline Phosphatase ,  Hydrolysis ,  antibody ,  *flow cytometry ,  *Fluorescence ,  optimization ,  *beta-Galactosidase ,  Endomembrane system ,  modification

JST classification (3)： Bioassay  (EA03060N) ,  Proteins and peptides in general  (EB03010N) ,  Analytical instruments  (CC02020S)

Terms in the title (9)： フローサイトメトリー ,  β-ガラクトシダーゼ ,  蛍光 ,  シグナル ,  増幅 ,  内因性 ,  活性 ,  酵素 ,  利点