J-GLOBAL ID:202010020197012953   Research Resource code:NBDC02277 Update date:Jul. 09, 2020

real-time quantitative PCR Primer Database: qPrimerDB

real-time quantitative PCR Primer Database: qPrimerDB
Owning Organization:
  • Southwest University
Resource classification: Data,Database
Tag (subject)  (3): Genome/Gene ,  DNA ,  Organism
Tag (data type)  (2): Sequence ,  Method
Species (8): Arabidopsis thaliana (NCBI Taxonomy ID: 3702) ,  Bombyx mori (NCBI Taxonomy ID: 7091) ,  Brassica (NCBI Taxonomy ID: 3705) ,  Drosophila (NCBI Taxonomy ID: 7215) ,  Homo sapiens (NCBI Taxonomy ID: 9606) ,  Mus musculus (NCBI Taxonomy ID: 10090) ,  Oryza (NCBI Taxonomy ID: 4527) ,  Schizosaccharomyces pombe (NCBI Taxonomy ID: 4896)
The qPrimerDB database stores information on qPCR primers. Accessible through a web front-end providing gene-specific and pre-computed primer pairs across 147 different organisms, including human, mouse, zebrafish, yeast, thale cress, rice, and maize. The qPrimerDB provides an interactive and information-rich web graphical interface to display search and BLAST results as table-based descriptions and associated links. In this database, we provide 3,331,426 of the best primer pairs for each gene based on primer pair coverage (PPC), as well as 47,726,569 alternative gene-specific primer pairs, which can be conveniently batch downloaded. We validated the specificity and efficiency of the qPCR primer pairs for 66 randomly selected genes in six different organisms through qPCR assays and gel electrophoresis. The qPrimerDB database represents a valuable, timesaving resource for gene expression analysis.
Source: FAIRsharing
Record maintainer: See the corresponding part in the FAIRsharing record
Record license: Creative Commons Attribution and Share-alike (CC-BY-SA) International 4.0 license

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