Rchr
J-GLOBAL ID：202101014515022044
Update date: Oct. 01, 2024
KOBAYASHI YOSHIAKI
KOBAYASHI YOSHIAKI
Affiliation and department：
Job title：
Assistant professor (special project faculty)
Homepage URL (1)：
https://www.bioreg.kyushu-u.ac.jp/labo/ged/
Research field (1)：
Molecular biology
Research keywords (7)：
核酸医薬
, マイクロRNA
, siRNA
, RNAサイレンシング
, RNA干渉
, 小分子RNA
, ノンコーディングRNA
Research theme for competitive and other funds (1)：
- 2021 - 2023 複雑な細胞環境におけるsiRNA-AGO複合体の分子動態の解明
Papers (10)：
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Kohei Nomura, Seongjin An, Yoshiaki Kobayashi, Jiro Kondo, Ting Shi, Hirotaka Murase, Kosuke Nakamoto, Yasuaki Kimura, Naoko Abe, Kumiko Ui-Tei, et al. Synthesis of 2′-formamidonucleoside phosphoramidites for suppressing the seed-based off-target effects of siRNAs. Nucleic Acids Research. 2024
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Shohei Maruyama, Takashi Kusakabe, Xinyi Zou, Yoshiaki Kobayashi, Yoshimasa Asano, Qingbo S. Wang, Kumiko Ui-Tei. SNPD-CRISPR: Single Nucleotide Polymorphism-Distinguishable Repression or Enhancement of a Target Gene Expression by CRISPR System. Methods in Molecular Biology. 2023. 49-62
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Lyu Fangjie, Seongjin An, Yoshiaki Kobayashi, Kohei Nomura, Rintaro Baba, Naoko Abe, Haruka Hiraoka, Fumitaka Hashiya, Zhaoma Shu, Kumiko Ui-Tei, et al. A 2'-modified uridine analog, 2'-O-(methylthiomethoxy)methyl uridine, for siRNA applications. Bioorganic & Medicinal Chemistry Letters. 2022. 74. 128939-128939
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Jiaxuan Song*, Yoshiaki Kobayashi* (*co-first author), Yoshimasa Asano, Atsushi Sato, Hiroaki Taniguchi, Kumiko Ui-Tei. Knockdown of 15-bp Deletion-Type v-raf Murine Sarcoma Viral Oncogene Homolog B1 mRNA in Pancreatic Ductal Adenocarcinoma Cells Repressed Cell Growth In Vitro and Tumor Volume In Vivo. Cancers. 2022. 14. 13. 3162-3162
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Kobayashi Y, Tian S, Ui-Tei K. Thermodynamic stabilities in the siRNA seed and non-seed regions regulate siRNA off-target effect in opposite direction. Research Aspects in Biological Science. 2022. 2. 117-130
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MISC (21)：
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小林 芳明, 浅野 吉政, 程 久美子. siRNAが働く仕組みと医薬品としての研究開発の現状-Mechanisms of RNA interference and the current status of research and development of siRNA drugs. 循環器内科 = Cardioangiology / 循環器内科編集委員会 編. 2023. 93. 3. 346-354
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大山隼礼, 小林芳明, 浅野吉政, 合山進, 程久美子, 程久美子. Development of siRNA specific for PIK3CA proto-oncogene with single-nucleotide mutation and its effect on cancer cell proliferation. 日本分子生物学会年会プログラム・要旨集(Web). 2023. 46th
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小野隆一, 小林芳明, 程久美子, 程久美子. Development of a CRISPR-Cas9 system that targets only harmful bacteria or metabolites in human gut microbiota. 日本分子生物学会年会プログラム・要旨集(Web). 2023. 46th
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小林芳明, 浅野吉政, 谷口博昭, 程久美子. Development and characterization of SNPD-siKRAS which specifically represses KRAS gene with single nucleotide mutation. 日本分子生物学会年会プログラム・要旨集(Web). 2023. 46th
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湯之前拓, 松井宏睦, 小林芳明, 程久美子, 程久美子. Development of a CRISPR system that distinguishes single nucleotide mutations in the TERT promoter to suppress carcinogenesis. 日本分子生物学会年会プログラム・要旨集(Web). 2023. 46th
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Patents (5)：
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RecQL1ヘリカーゼ遺伝子を標的とするsiRNA
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RNA分子、キメラ型NA分子、二本鎖RNA分子、および二本鎖キメラ型NA分子
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RNA分子、キメラ型NA分子、二本鎖RNA分子、および二本鎖キメラ型NA分子
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RNA分子、キメラ型NA分子、二本鎖RNA分子、および二本鎖キメラ型NA分子
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RNA干渉におけるsiRNAのオフターゲット効果を回避する合成核酸2'-formamide
Books (3)：
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核酸医薬 : モダリティ・合成・分析・DDSの最新動向
エヌ・ティー・エス 2024 ISBN:9784860438869
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核酸医薬・mRNA医薬の製造分析の基礎と基盤技術開発
シーエムシー・リサーチ 2023 ISBN:9784910581347
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核酸医薬 : 本領を発揮する創薬モダリティ
羊土社 2021 ISBN:9784758103985
Lectures and oral presentations (45)：
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Thermodynamic and Structural Analysis of the Effects of Chemical Modifications in the siRNA Seed Region on Off-Target Effects.
(2024)
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Development and validation of a single nucleotide mutation-distinguishable siRNA for PIK3CA (SNPD-siPIK3CA).
(2024)
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2’位化学修飾核酸の導入による siRNA の標的特異性の向上
(第 9 回日本核酸医薬学会 若手シンポジウム 2024)
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Thermodynamic and Structural Impacts of Chemical Modifications in the siRNA Seed Region on Off-Target Effects.
(2024)
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siRNAs discriminate single nucleotide differences in PIK3CA.
(2024)
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Education (4)：
- 2018 - 2021 The University of Tokyo Graduate School of Science Doctoral Program Department of Biological Sciences
- 2016 - 2018 The University of Tokyo Graduate School of Science Department of Biological Sciences
- 2012 - 2016 Soka University
- 2009 - 2012 鳥取県立鳥取西高等学校 普通科
Professional career (1)：
Work history (2)：
- 2024/04 - 現在 Kyushu University Division of Gene Expression Dynamics, Medical Research Center Initiative for High Depth Omics, Medical Institute of Bioregulation Assistant professor (special project faculty)
- 2021/04 - 2024/03 The University of Tokyo Graduate School of Science Department of Biological Sciences Research Fellow
Awards (1)：
- 2019/07 - 優秀発表者賞(川原賞) Systematic 2'-O-methyl modification revealed that siRNA seed region consists of two functionally different regions
Association Membership(s) (3)：
The RNA Society of Japan
, Nucleic Acids Therapeutics Society of Japan
, The Molecular Biology Society of Japan
