Art

J-GLOBAL ID：202102212597699473   Reference number：21A0219785

Quantitative localization of neurotransmitter receptors and ion channels on the neuronal plasma membrane by SDS-digested Freeze-fracture Replica Labeling

SDS-FRL法を用いたシナプス伝達関連膜分子の定量的局在解析

Author (2)： KURODA KAZUKI (福井大 医 脳形態機能学分野) ,  FUKAZAWA YUGO (福井大 医 脳形態機能学分野)
Material： 生化学
Volume： 92  Issue： 6  Page： 844-849  Publication year： Dec. 25, 2020 
JST Material Number： G0184A  ISSN： 0037-1017  CODEN： SEIKAQ  Document type： Article
Article type： 文献レビュー  Country of issue： Japan (JPN)  Language： JAPANESE (JA)

Thesaurus term： cryogenic crushing ,...
Semi thesaurus term： *cellular signal transduction ,...

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JST classification (3)： Microscopic methods of living organisms  (EA03050C) ,  Central nervous system  (EJ12030F) ,  Basic neurology  (GN01020W)

Substance index (1)： Dodecyl sodium sulfate

Reference (15)：

• Masugi-Tokita, M. & Shigemoto, R. (2007) High-resolution quantitative visualization of glutamate and GABA receptors at central synapses. Curr. Opin. Neurobiol., 17, 387-393.
• Fujimoto, K. (1995) Freeze-fracture replica electron microscopy combined with SDS digestion for cytochemical labeling of integral membrane proteins. Application to the immunogold labeling of intercellular junctional complexes. J. Cell Sci., 108, 3443-3449.
• Tanaka, J., Matsuzaki, M., Tarusawa, E., Momiyama, A., Molnar, E., Kasai, H., & Shigemoto, R. (2005) Number and density of AMPA receptors in single synapses in immature cerebellum. J. Neurosci., 25, 799-807.
• Indriati, D.W., Kamasawa, N., Matsui, K., Meredith, A.L., Watanabe, M., & Shigemoto, R. (2013) Quantitative localization of Cav2.1 (P/Q-type) voltage-dependent calcium channels in Purkinje cells: somatodendritic gradient and distinct somatic coclustering with calcium-activated potassium channels. J. Neurosci., 33, 3668-3678.
• Hagiwara, A., Fukazawa, Y., Deguchi-Tawarada, M., Ohtsuka, T., & Shigemoto, R. (2005) Differential distribution of release-related proteins in the hippocampal CA3 area as revealed by freeze-fracture replica labeling. J. Comp. Neurol., 489, 195-216.

Terms in the title (5)： SDS ,  シナプス伝達 ,  分子 ,  定量 ,  解析