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J-GLOBAL ID：201902223556947210   整理番号：19A1281159

宿主標的化抗ウイルス戦略としてのZikaウイルスの非構造蛋白質5のSUMO修飾の標的化【JST・京大機械翻訳】

Targeting SUMO Modification of the Non-Structural Protein 5 of Zika Virus as a Host-Targeting Antiviral Strategy

著者 (28件)： Zhu Zheng (State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Zhu Zheng (Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Chu Hin (State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Chu Hin (Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Wen Lei (Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Yuan Shuofeng (State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Yuan Shuofeng (Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Chik Kenn Ka-Heng (Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Yuen Terrence Tsz-Tai (Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Yip Cyril Chik-Yan (Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Wang Dong (Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Zhou Jie (State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Zhou Jie (Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Yin Feifei (Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, Hainan, and The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Yin Feifei (Department of Pathogen Biology, Hainan Medical University, Haikou, Hainan 571101, China) ,  Yin Feifei (Key Laboratory of Translational Tropical Medicine, Hainan Medical University, Haikou, Hainan 571101, China) ,  Jin Dong-Yan (School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Kok Kin-Hang (State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Kok Kin-Hang (Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Yuen Kwok-Yung (State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Yuen Kwok-Yung (Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Yuen Kwok-Yung (Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, Hainan, and The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Yuen Kwok-Yung (Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Yuen Kwok-Yung (The Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Chan Jasper Fuk-Woo (State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Chan Jasper Fuk-Woo (Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Chan Jasper Fuk-Woo (Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, Hainan, and The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China) ,  Chan Jasper Fuk-Woo (Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China)
資料名： International Journal of Molecular Sciences (Web)  (International Journal of Molecular Sciences (Web))
巻： 20  号： 2  ページ： 392  発行年： 2019年 
JST資料番号： U7038A  ISSN： 1422-0067  CODEN： IJMCFK  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： スイス (CHE)  言語： 英語 (EN)

抄録/ポイント： 宿主またはウイルス蛋白質の翻訳後修飾はウイルス複製を支援し,宿主免疫応答を打ち消すためにウイルスにより利用される重要な戦略である。スモイル化は,小ユビキチン様修飾因子(SUMO)蛋白質と呼ばれるユビキチン様蛋白質ファミリーにより仲介される翻訳後修飾過程である。78の代表的フラビウイルスの多重配列アラインメントは,ほとんど(72/78,92.3%)が非構造5(NS5)蛋白質N末端ドメインにおいて推定SUMO相互作用モチーフ(SIM)を有することを示した。推定SIMは,414の前流行性および流行性Zikaウイルス(ZIKV)株の間で高度に保存されており,それらの全てはVIDL(327/414,79.0%)またはVVDL(87/414,21.0%)の推定SIMコアアミノ酸配列を有していた。分子ドッキングは,疎水性SIMコア残基がSUMO-1蛋白質の2本鎖に結合し,コアに隣接する酸性残基がSUMO蛋白質の塩基性表面との相互作用を介して結合を強化することを予測した。SUMO阻害剤2-D08はフラビウイルスの複製を有意に減少させ,in vitroでZIKV誘導細胞変性効果に対して細胞を保護した。SIM変異ZIKV NS5はI型インターフェロンシグナリングを効率的に抑制できなかった。全体として,これらの知見はウイルスNS5蛋白質のSUMO修飾がウイルス複製を増強し,宿主抗ウイルス応答を抑制するフラビウイルス間の進化的に保存された翻訳後修飾過程であることを示唆する。Copyright 2019 The Author(s) All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 相互作用 ,  ユビキチン ,  翻訳後修飾 ,  ウイルス複製 ,  *ターゲティング ,  インターフェロン ,  変異 ,  フラビウイルス ,  *タンパク質 ,  アミノ酸配列 ,  in vitro実験
準シソーラス用語： 抗ウイルス応答 ,  残基 ,  SUMO化 ,  *SUMO , 【Automatic Indexing@JST】

著者キーワード (8件)： 抗ウイルス ,  フラビウイルス ,  阻害剤 ,  インターフェロン ,  NS5 ,  翻訳後修飾 ,  SUMO ,  Zika

分類 (1件)： 遺伝子発現  (EC02040Q)

引用文献 (44件)：

• Zhu, Z.; Chan, J.F.; Tee, K.M.; Choi, G.K.; Lau, S.K.; Woo, P.C.; Tse, H.; Yuen, K.Y. Comparative genomic analysis of pre-epidemic and epidemic Zika virus strains for virological factors potentially associated with the rapidly expanding epidemic. Emerg. Microbes Infect. 2016, 5, e22.
• Duffy, M.R.; Chen, T.H.; Hancock, W.T.; Powers, A.M.; Kool, J.L.; Lanciotti, R.S.; Pretrick, M.; Marfel, M.; Holzbauer, S.; Dubray, C.; et al. Zika virus outbreak on Yap Island, Federated States of Micronesia. N. Engl. J. Med. 2009, 360, 2536-2543.
• Chan, J.F.; Choi, G.K.; Yip, C.C.; Cheng, V.C.; Yuen, K.Y. Zika fever and congenital Zika syndrome: An unexpected emerging arboviral disease. J. Infect. 2016, 72, 507-524.
• Musso, D.; Gubler, D.J. Zika Virus. Clin. Microbiol. Rev. 2016, 29, 487-524.
• [Green Version] World Health Organization. Zika Situation Report. 10 March 2017. Available online: http://who.int/emergencies/zika-virus/situation-report/10-march-2017/en/ (Accessed on 1 July 2018).

タイトルに関連する用語 (7件)： 宿主 ,  標的化 ,  ウイルス ,  戦略 ,  構造蛋白質 ,  SUMO ,  修飾