文献

J-GLOBAL ID：202002216537804745   整理番号：20A2649605

神経内分泌新生物のオルガノイドバイオバンクは遺伝子型-表現型マッピングを可能にする【JST・京大機械翻訳】

An Organoid Biobank of Neuroendocrine Neoplasms Enables Genotype-Phenotype Mapping

著者 (49件)： Kawasaki Kenta (Department of Organoid Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Kawasaki Kenta (Department of Gastroenterology, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Toshimitsu Kohta (Department of Organoid Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Toshimitsu Kohta (Department of Gastroenterology, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Matano Mami (Department of Organoid Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Fujita Masashi (Laboratory for Cancer Genomics, RIKEN Center for Integrative Medical Sciences, Yokohama 230-0045, Japan) ,  Fujii Masayuki (Department of Organoid Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Fujii Masayuki (Deparment of Surgical Oncology, The University of Tokyo, Tokyo 113-8654, Japan) ,  Togasaki Kazuhiro (Department of Organoid Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Togasaki Kazuhiro (Department of Gastroenterology, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Ebisudani Toshiki (Department of Organoid Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Ebisudani Toshiki (Department of Pulmonary Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Shimokawa Mariko (Department of Organoid Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Takano Ai (Department of Organoid Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Takahashi Sirirat (Department of Organoid Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Ohta Yuki (Department of Organoid Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Nanki Kosaku (Department of Organoid Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Nanki Kosaku (Department of Gastroenterology, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Igarashi Ryo (Department of Organoid Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Igarashi Ryo (Department of Gastroenterology, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Ishimaru Kazuhiro (Department of Organoid Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Ishimaru Kazuhiro (Deparment of Surgical Oncology, The University of Tokyo, Tokyo 113-8654, Japan) ,  Ishida Hiroki (Department of Organoid Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Ishida Hiroki (Department of Surgery, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Sukawa Yasutaka (Department of Organoid Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Sukawa Yasutaka (Department of Gastroenterology, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Sugimoto Shinya (Department of Organoid Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Sugimoto Shinya (Department of Gastroenterology, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Saito Yoshimasa (Department of Gastroenterology, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Saito Yoshimasa (Division of Pharmacotherapeutics, Keio University Faculty of Pharmacy, Tokyo 105-8512, Japan) ,  Maejima Kazuhiro (Laboratory for Cancer Genomics, RIKEN Center for Integrative Medical Sciences, Yokohama 230-0045, Japan) ,  Sasagawa Shota (Laboratory for Cancer Genomics, RIKEN Center for Integrative Medical Sciences, Yokohama 230-0045, Japan) ,  Lee Hwajin (Biomedical Knowledge Engineering Laboratory, Seoul National University, Seoul 08826, South Korea) ,  Kim Hong-Gee (Biomedical Knowledge Engineering Laboratory, Seoul National University, Seoul 08826, South Korea) ,  Ha Kyungsik (Biomedical Knowledge Engineering Laboratory, Seoul National University, Seoul 08826, South Korea) ,  Hamamoto Junko (Department of Pulmonary Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Fukunaga Koichi (Department of Pulmonary Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Maekawa Aya (Department of Hepatobiliary and Pancreatic Surgery, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo 113-8510, Japan) ,  Tanabe Minoru (Department of Hepatobiliary and Pancreatic Surgery, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo 113-8510, Japan) ,  Ishihara Soichiro (Deparment of Surgical Oncology, The University of Tokyo, Tokyo 113-8654, Japan) ,  Hamamoto Yasuo (Department of Gastroenterology, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Yasuda Hiroyuki (Department of Pulmonary Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Sekine Shigeki (Division of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo 104-0045, Japan) ,  Kudo Atsushi (Department of Hepatobiliary and Pancreatic Surgery, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo 113-8510, Japan) ,  Kitagawa Yuko (Department of Surgery, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Kanai Takanori (Department of Gastroenterology, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Nakagawa Hidewaki (Laboratory for Cancer Genomics, RIKEN Center for Integrative Medical Sciences, Yokohama 230-0045, Japan) ,  Sato Toshiro (Department of Organoid Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan) ,  Sato Toshiro (Department of Gastroenterology, Keio University School of Medicine, Tokyo 160-8582, Japan)
資料名： Cell (Cambridge, Mass.)  (Cell (Cambridge, Mass.))
巻： 183  号： 5  ページ： 1420-1435.e21  発行年： 2020年 
JST資料番号： A0707B  ISSN： 0092-8674  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： アメリカ合衆国 (USA)  言語： 英語 (EN)

抄録/ポイント： 神経内分泌腫瘍と神経内分泌癌(NEC)から成る胃腸膵臓(GEP)神経内分泌腫瘍(NEN)は,その希少性のため致死性であるが,未調査の疾患である。GEP-NENの臨床関連モデルの不足を埋めるために,ここではNENオルガノイドの25系統を確立し,それらの包括的分子特性化を行った。GEP-NENオルガノイドは,元の腫瘍の病理組織学的および機能的表現型を再現した。全ゲノム配列決定は,GEP-NECにおけるTP53とRB1の頻繁な遺伝的変化,およびGEP-NENにおけるヘテロ接合性の特徴的な染色体全体の損失を明らかにした。トランスクリプトーム解析により,異なる転写因子の発現により識別される分子サブタイプを同定した。GEP-NENオルガノイドは,遺伝子変異に関係なく幹細胞ニッチから独立性を獲得した。TP53とRB1の化合物ノックアウトは,重要な転写因子の過剰発現と共に,GEP-NEN生物学に適合する正常な結腸上皮表現型に付与された。まとめると,著者らの研究はGEP-NENの遺伝的理解を提供するだけでなく,その遺伝学と生物学的表現型を接続する。Copyright 2020 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： *遺伝 ,  *遺伝子型 ,  染色体 ,  結腸 ,  *腫瘍 ,  上皮 ,  *表現型 ,  *神経分泌 ,  転写因子 ,  遺伝子変異
準シソーラス用語： *オルガノイド ,  トランスクリプトーム解析 ,  ゲノム配列 ,  幹細胞ニッチ ,  過剰発現 , 【Automatic Indexing@JST】

著者キーワード (10件)： CRISPR-Cas9 ,  NKX2-5 ,  ガストリノーマ ,  3D培養 ,  系統再プログラミング ,  エピゲノム ,  幹細胞ニッチ ,  転写因子 ,  融合遺伝子 ,  マルチオミクス

分類 (2件)： 細胞生理一般  (EF02010S) ,  腫ようの化学・生化学・病理学  (GE02030N)

タイトルに関連する用語 (5件)： 神経内分泌 ,  新生物 ,  遺伝子型 ,  表現型 ,  マッピング