文献

J-GLOBAL ID：202102220689064475   整理番号：21A0023612

ABCA8はmiR-374b-5pにより調節され,ERK/ZEB1経路を通して肝細胞癌の増殖と転移を阻害する【JST・京大機械翻訳】

ABCA8 is regulated by miR-374b-5p and inhibits proliferation and metastasis of hepatocellular carcinoma through the ERK/ZEB1 pathway

著者 (34件)： Cui Yifeng (Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China) ,  Cui Yifeng (Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, China) ,  Liang Shuhang (Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China) ,  Liang Shuhang (Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, China) ,  Zhang Shugeng (Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China) ,  Zhang Shugeng (Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, China) ,  Zhang Congyi (Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China) ,  Zhang Congyi (Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, China) ,  Zhao Yunzheng (Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China) ,  Zhao Yunzheng (Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, China) ,  Wu Dehai (Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China) ,  Wu Dehai (Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, China) ,  Wang Jiabei (Department of Hepatobiliary Surgery, The First Affiliated Hospital of University of Science and Technology of China, Hefei, China) ,  Song Ruipeng (Department of Hepatobiliary Surgery, The First Affiliated Hospital of University of Science and Technology of China, Hefei, China) ,  Wang Jizhou (Department of Hepatobiliary Surgery, The First Affiliated Hospital of University of Science and Technology of China, Hefei, China) ,  Yin Dalong (Department of Hepatobiliary Surgery, The First Affiliated Hospital of University of Science and Technology of China, Hefei, China) ,  Liu Yao (Department of Hepatobiliary Surgery, The First Affiliated Hospital of University of Science and Technology of China, Hefei, China) ,  Pan Shangha (Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, China) ,  Liu Xirui (Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China) ,  Liu Xirui (Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, China) ,  Wang Yan (Department of Colorectal Surgery, Harbin Medical University Cancer Hospital, Harbin, China) ,  Han Jihua (Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China) ,  Han Jihua (Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, China) ,  Meng Fanzheng (Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China) ,  Meng Fanzheng (Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, China) ,  Zhang Bo (Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China) ,  Zhang Bo (Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, China) ,  Guo Hongrui (Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China) ,  Guo Hongrui (Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, China) ,  Lu Zhaoyang (Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China) ,  Lu Zhaoyang (Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, China) ,  Liu Lianxin (Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China) ,  Liu Lianxin (Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, China) ,  Liu Lianxin (Department of Hepatobiliary Surgery, The First Affiliated Hospital of University of Science and Technology of China, Hefei, China)
資料名： Journal of Experimental & Clinical Cancer Research (Web)  (Journal of Experimental & Clinical Cancer Research (Web))
巻： 39  号： 1  ページ： 1-14  発行年： 2020年 
JST資料番号： U7514A  ISSN： 1756-9966  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： イギリス (GBR)  言語： 英語 (EN)

抄録/ポイント： ATP結合カセットサブファミリーAメンバー8(ABCA8)はATP結合カセット(ABC)輸送体スーパーファミリーに属する。ABCA8はコレステロールや薬物流出などの有機物の輸送に関与する膜貫通輸送体である。ABCA1のようなABCサブファミリーのいくつかのメンバーは癌発生を阻害する可能性がある。しかし,癌活性化過程におけるABCA8の機構は,まだあいまいである。ヒト肝細胞癌(HCC)組織および細胞株におけるABCA8の発現を,qPCR,免疫ブロッティングおよび免疫組織化学染色を用いて調べた。HCCの増殖と転移に対するABCA8の影響をin vitroとin vivo機能試験を用いて調べた。ルシフェラーゼレポーターアッセイを行い,マイクロRNA-374b-5p(miR-374b-5p)とABCA83’-非翻訳領域(UTR)との結合を調べた。ABCA8はHCCで頻繁にダウンレギュレートされ,このダウンレギュレーションは予後と負に相関した。ABCA8の過剰発現はHCCにおける増殖と転移を阻害したが,ABCA8のノックダウンはin vivoとin vitroの両方で抗美的作用を発揮した。ABCA8はmiR-374b-5pによって下方制御された;このダウンレギュレーションは,ERK/ZEB1シグナリング経路を介し,間葉への上皮形質転換を誘導し,HCC進行を促進する。HCCにおけるABCA8の予後的価値を曝露し,HCC腫瘍形成と転移のABCA8調節阻害における新規経路を明らかにした。これら所見は,ABCA8とmiR-374b-5pの調節を介し,HCCに対する新しい標的化治療を生じる。Copyright 2021 The Author(s) All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 上皮 ,  ヒト ,  ルシフェラーゼ ,  予後 ,  間充織 ,  ターゲティング ,  *肝細胞癌 ,  ダウンレギュレーション ,  in vitro実験 ,  定量的PCR法 ,  ステロール ,  脂環式アルコール
準シソーラス用語： マイクロRNA ,  非翻訳領域 ,  レポーターアッセイ ,  過剰発現 , 【Automatic Indexing@JST】

著者キーワード (4件)： 肝細胞癌 ,  ATP結合カセットサブファミリーaメンバー8 ,  上皮から間葉への移行 ,  治療標的

分類 (3件)： 腫ようの化学・生化学・病理学  (GE02030N) ,  細胞膜の輸送  (EF04020R) ,  消化器の腫よう  (GH04000D)

物質索引 (1件)： コレステロール

引用文献 (42件)：

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タイトルに関連する用語 (8件)： MIR ,  調節 ,  ERK ,  ZEB1 ,  経路 ,  肝細胞癌 ,  増殖 ,  阻害